外源施加GA和6-BA对棉纤维发育过程中细胞凋亡的影响

Effection of Add GA and 6-BA towards Apoptosis in the Development of Cotton Fiber

主要通过对棉纤维发育过程中的细胞总DNA的生化特征和SOD、MDA和可溶性蛋白三种生理指标的研究,确定了棉纤维发育过程中细胞凋亡的大致时间。通过施加不同浓度的GA和6-BA,我们发现10mg/L的GA能使细胞凋亡出现的时间提前3d左右,而5mmol/L的6-BA能使细胞凋亡出现的时间推迟3d左右,高于以上浓度的植物生长调节剂可能会由于刺激过度,而使细胞的总DNA在凝胶电泳时呈现模糊状条带。以上实验,对未来利用分子生物学手段改善棉纤维品质具有重要的意义。

This paper tell us about when apoptosis happens in the development of cotton fiber through research towards cell genomic DNA and three physiological measurement SOD,MDA and soluble protein.Through add GA and 6-BA,we found that 10mg/L GA made apoptosis ahead of time about 3 days,and 5mmol/L 6-BA made apoptosis delayed about 3 days,and above those concentrations,genomic DNA was degraded seriously.This experiment will play a very important role in improving cotton fiber＇s character by molecular biological measure in the future.