丹参酮微乳的制备及大鼠在体肠吸收

Preparation of tanshinone microemulsion and its absorption in rat intestine in situ

目的:制备丹参酮微乳(Tan-ME),并考察其性质及大鼠在体肠吸收特性。方法:Zetapals多功能电位／粒度分析仪测定Tan-ME的粒度及其分布,原子力显徽镜观察微乳粒子形态。建立测定肠回流液中丹参酮Ⅱ_A的HPLC测定方法。结果:Tan-ME乳滴平均粒径为(32．25±6．59)nm,粒度均匀。肠吸收实验结果表明,Tan-ME能促进丹参酮在大鼠小肠的吸收,但Tan-ME吸收速率受Tan-ME水相含量影响较大。结论:Tan-ME制剂能够促进丹参酮在大鼠小肠的吸收,提高丹参酮的生物利用度。

Objective： To prepare tanshinone microemulsion （Tan-ME） and investigate its properities and the absorption character in rat intestine in situ. Method： The microemulsions were prepared and characterized using Zetapals Zeta potential/particle size analysis and atomic force microscope. A HPLC method for determination of tanshinone Ⅱ A in the intestinal flux was established. Resuit： The Tan-ME was fine droplet with an average droplet size of （32. 25 ±6. 59） nm. The results of Tan-ME absorption in small intestinal indicated that Tan-ME could improve the absorption of tanshinone Ⅱ A in rat small intestine, but the absorption coefficient of Tan-ME, Ka was influenced by the ratio of water-phase in Tan-ME. Conclusion： Tan-ME could improve the absorption of tanshinone Ⅱ A in rat small intestine. Thus bioavailability of tanshinone was improved.