大黄(庶虫)虫丸拆方抗家兔动脉粥样硬化的机制研究

Study on anti-atherosclerotic mechanisms of divided functional recipes of Dahuang Zhechong pill in rabbits

目的:研究大黄(庶虫)虫丸拆方抗家兔动脉粥样硬化(AS)的作用机制。方法:采用免疫性内皮损伤合并高脂饲料喂养建立家兔AS模型。将雄性新西兰家兔随机分为9组,正常对照组、模型对照组、丹参阳性药对照组,大黄(庶虫)虫丸拆方1,2,3号各设低、高2个剂量组。灌胃给药60 d后处死,测定各组动物的血脂、血清丙二醛(MDA)和一氧化氮(NO)、超氧化物歧化酶(SOD)、血浆内皮素(ET)、血管壁羟脯氨酸及蛋白的含量。结果:与模型对照组相比,大黄(庶虫)虫丸拆方各组对AS模型家兔的血脂水平无明显影响,但均可降低血清MDA和ET水平(P＜0．05),升高SOD活性和NO含量(P＜0．05),并减少血管壁羟脯氨酸及蛋白含量(P＜0．05)。结论:大黄(庶虫)虫丸拆方各功效组分通过抗脂质过氧化、保护内皮功能、减少血管壁胶原及蛋白的合成而减少细胞外基质(ECM)形成等非降脂的作用机制,以防治AS的发生与发展,其中以拆方1号作用最显著,这可能与其疏通经络、活血化瘀、破瘀生新等功效有关。

Objective： To study anti-atherosclerotic mechanisms of divided functional recipes of Dahuang Zhechong pill （DHZCP） in rabbits. Method： The atherosclerotic rabbit model was established by high fat feeding combined with immune endothelial injury. Male New Zealand rabbits were divided into 9 groups： normal control group, model control group, Danshen positive control group, and 6 DHZCP-divided groups including divided functional recipes No. 1,2, 3 with low and high doses for each divided recipe. After intragastric administration for 60 days, blood lipids and serum MDA and NO levels and SOD activity and plasma ET concentration, and contents of hydroxyproline and proteins in the vascular wall were determined. Result： Compared with the model group, the level of blood lipids did not significantly change, serum MDA and ET levels, and the contents of hydroxyproline and proteins in the vascular wall significantly decreased （ P 〈 0.05 ）, and SOD activity and NO level increased in the divided functional recipes （ all P 〈 0. 05）. Conclusion： The divided functional recipes of DHZCP can inhibit development of atherosclerosis via a non-lowering lipid mechanisms, including anti-peroxidation of lipids, protection of endothelial function, and decrease of formation of extracellular matrix by reducing synthesis of collage and protein on the vascular wall. Among them, the divided functional recipe No. 1 exhibits the most obvious effect.