马鬃蛇对去势大鼠垂体雄激素受体水平的影响

Effects of Calotes versicolor on adenohypophysis androgen receptor level in mature castrated male rats

目的:研究马鬃蛇石油醚提取物(CVPE)对去势大鼠垂体雄激素受体水平的影响。方法:大鼠麻醉,无菌下摘除双侧睾丸及附睾。术后第14天随机分为4组。1组为模型组,灌胃(ig)生理盐水,2组为CVPE低剂量组2 g·kg^(-1),3组为高剂量组4 g·kg^(-1),4组为阳性药、组皮下注射(ih)丙酸睾丸素20 mg·kg^(-1)。连续每天1次灌胃CVPE,21 d后采用免疫组化法测定垂体雄激素受体(AR)水平,用放射免疫法测定血清睾酮水平。结果:给药组(高、低剂量组)和阳性药组的雄激素受体阳性细胞数均明显多于模型组,并显著升高血清睾酮水平,与模型组比较有显著性差异(P_低＜0．05,P_高＜0．01)。结论:CVPE可提高成年去势大鼠的垂体雄激素受体及血清睾酮水平,提示其可能通过垂体发挥作用。

Objective： To study the effects of C. versicolor petroleum ether extracts （CVPE） on the adenohypophysis androgen receptor level in mature castrated male rats. Method： All the rats in experiment were anesthetized for bilateral testicular and epididymis removal under sterile condition. The rats were randomized into four groups on the 14 th day after operation. The first group was intragastric physiological saline for castratered control group. The second group was intragastric CVPE 2 g ·kg^-1 for low-dose group. The third group was high-dose group by giving CVPE 4 g ·kg^-1. The fourth group was injected hypodermic testosterone propionate for positive-effect drug treatment group. The drug was given orally to animals one time a day successively for 21 days. The androgen receptor （AR） in adenohypophysis of mature castrated male rats was determined by the immunohistochemistry method and the level of serum testosterone （T） were determined by the radio-immunoassay after ig CVPE for 21 days. Result： The immunohistochemistry results showed that positive cell numbers of androgen receptor in positive control and each CVPE groups were more than those in the castrated control group. The serum T level was increased greatly in mature castrated male rats treated with CVPE compared with the control group （P 〈0. 05, P 〈0. 01 ）. Conclusion： The results show that CVPE can increase the adenohypophysis androgen receptor and serum T level in mature castrated male rats. It is indicated that CVPE has the effects on the hypophysis function.