^(137)Csγ射线对白膜不同成分的影响

Effect of ^(137)Cs-irradiation on the components of buffy coat

目的137Csγ射线对白膜不同成分的影响。方法MTT法测辐照前后淋巴细胞的增殖情况;用荧光素FITC标记DH5α细菌,FCM分析粒细胞荧光强度的变化;用细胞色素C还原法检测中性粒细胞O2-的生成以及用ELISA法测单核细胞辐照前后细胞因子的分泌情况。结果25 Gy能完全灭活T细胞;辐照后粒细胞离体12h后有明显凋亡出现,但辐照前后粒细胞吞噬DH5α的能力无明显改变;而粒细胞释放的O2-在辐照后有明显升高;单核细胞经辐照后仍能分泌细胞因子,且IFN-γ的分泌量较未照组有所下降,而TNF-α的分泌量随着时间的推移呈逐渐升高趋势。结论γ射线对白膜中各成分都有不同程度的影响。分离后的粒细胞应尽早输注,由细胞因子参与的临床输血问题以及由此带来的风险,应当引起关注。

Objective To investigate the changes of huffy coat components after 25Gy ^137Cs-irradiation. Methods Lymphocyte proliferation was detected by MTT, the fluorescent intensity of neutrophils was measured as phagocytotic rate by flow cytometry, O2^- release was determined by cytochrome C reduction, and cytokine production preand post-irradiation was detected by ELISA. Results 25Gy is adequate to inactivate T cells. The percentages of phagocytosis of E. coli before and after irradiation were of no .significant difference. The release of O2 - increased sig nificantly and monocytes maintained the ability to produce cytokines after irradiation. Conclusion Radiation does affect the components of huffy coat, and neutrophils isolated should be transfused as early as possible .