DiI顺行追踪大鼠皮质脊髓束

DiI ANTEROGRADE-TRACING THE CORTICOSPINAL TRACT OF THE RAT

目的用DiI追踪大鼠皮质脊髓束,探讨DiI在皮质脊髓束顺行追踪中的应用效果。方法Wistar大鼠24只,随机分为DiI组和溃变组,于DiI组感觉运动区皮质表面涂布5%DiI;对溃变组则损毁相应的感觉运动区皮层。结果DiI组术后存活5d的鼠,仅在端脑和间脑区见红色荧光标记的纤维,在术后存活10d、15d、20d的鼠脑及脊髓各节段内均可见红色荧光标记的皮质脊髓束纤维;溃变组在脊髓节段可观察到明显的溃变纤维,以术后存活5d最为显著,在脊髓以上节段未见明显溃变纤维。结论采用DiI活体顺行追踪大鼠皮质脊髓束的方法简便易行,对于皮质脊髓束的示踪研究,尤其中脑以下节段的显示具有比较好的应用价值。

Objective To study the effect of anterograde-tracing the corticospinal tract of the rat using DiI a tracer. Methods Twenty-four Wistar rats were randomly divided into DiI group and degeneration group. 5 % DiI were stained on the motorsensor cortex of the DiI group rats ; the corresponding cortex of the degeneration group were destroyed. After 5d, 10d, 15d and 20d,the rats were killed respectively. The brains and spinal cords were sliced and observed. Results In the 5d DiI group, labelled fibers were only observed both in telencephalon and diencephalon, and in 10d, 15d and 20d groups, corticospinal tracts were well labelled through the brain and spinal cord. In the degeneration group, black degenerating fibers of the spinal cords were well demonstrated, especially in the 5d group. However, above the spinal cords, no obvious degenerating fibers could be found. Conclusion It＇s a simple, convenient and valuable method, using DiI as an anterograde tracer, to trace corticospinal tract, especially the part through midbrain to spinal cord.