中国HCV/HIV合并感染者淋巴细胞CD25表达和血清IP-10的研究

Surface Expression of CD25 from PBMC and Levels of Serum IP-10 in HCV/HIV Co-infected Patients in China

目的研究淋巴细胞表面受体CD25和血清干扰素诱导蛋白10(IP-10),在丙型肝炎病毒(HCV)单纯感染,人类免疫缺陷病毒(HIV)单纯感染和HCV/HIV合并感染中的表达及意义。方法采用流式细胞术,检测HCV感染组(n=21)、HIV感染组(n=14)、HCV/HIV感染组(n=28)及正常对照组(n=30),T淋巴细胞表面CD25的表达,ELISA方法检测血清IP-10含量。结果血清IP-10水平在HCV感染组、HIV感染组和HCV/HIV合并感染组都明显升高,HIV感染组及HCV/HIV合并感染组CD4+T细胞表面CD25表达显著降低(P<0.01);HCV感染组CD4+T细胞表面CD25表达轻度升高,但差异无统计学意义。结论中国HCV/HIV合并感染与CD4+T细胞CD25表达及血清IP-10水平密切相关。

OBJECTIVE To investigate expression and significance of CD25 and IP-10 in HCV mono-infection, HIV mono-infection and HCV/HIV co-infection. METHODS PBMC were isolated from patients with HCV infection （n= 21）, HIV infection （n = 14）, HCV/HIV co-infection （ n = 28） and normal control （ n = 30）. The surface expression of CD25 on T lymphocytes was determined by flow cytometry. The serum IP-10 levels were measured by ELISA, RESULTS Increased levels of IP-10 were detected in HCV infection, HIV infection and HCV/HIV co-infection. A lower proportion of CD4^＋ CD25^＋ T lymphocytes was found in HIV infection and HCV/HIV co-infection （P〈0.01）, while slight but not significant increased proportion of CD4^＋ CD25^＋ T lymphocytes was detected in HCV infection. CONCLUSIONS Chemokine IP-10 and lymphocyte surface receptor CD25 are correlative with HCV and HIV infection closely,