泌尿生殖道解脲脲支原体感染的分子流行病学研究

Molecular Epidemiology of Ureaplasma urealyticum in Patients with Urogenital Infections

目的了解粤东地区泌尿生殖道感染人群中解脲脲支原体的感染及其型别分布。方法用液体选择培养基进行临床标本的初筛,再用PCR法进行分群和分型。结果1 790例临床标本中解脲脲支原体阳性670例(37.43%),对392例阳性培养物进行分群,生物1群阳性率为71.94%;生物2群阳性率为29.85%;对264例单纯生物1群阳性标本进行PCR分型结果血清型1有20例(7.58%),血清型3或14有139例(52.65%),血清型6有51例(19.32%)并有大量混合感染;对99例单纯生物2群感染标本的PCR分型结果,基因型3(血清型7、11)最低,基因型1和2感染率较高。结论粤东地区泌尿生殖道感染人群中,解脲脲支原体以生物1群中的血清型3和血清型6单纯感染为主,但不同血清型或基因型在该地区的分布有一定的特征性。

OBJECTIVE To investigate the Ureaplasrna urealyticum infection in patients with urogenital infections and the distribution of its serotypes and genotypes. METHODS The clinical samples were firstly screened with B- Merieux mycoplasma ID2 culture and identification kit, then the positive cultural samples were subtyped by PCR method. RESULTS The positive rate of U. urealytlcum with cultural kit was 37. 43% （670/1790）. The 392 positive cultural samples were subtyped with PCR, the positive rate of U. parvurn was 71.94% and the positive rate of U. urealyticum was 29. 85%. The serotypes of 264 U. parvum positive samples were identified with PCR, there were 20 samples with serovar 1 （7.58%）, 139 samples with serovar 3 or 14 （52. 65%）, and 51 samples with serovar 6 （19.32 %）. Ninety nine positive samples with U. urealyticum were genotyped, the results showed that the rate of genotype 3 （serovar 7 or 11） was lower than genotypes 1 and 2. CONCLUSIONS PCR identification and subtyping of Ureaplasma in patients with urogenital infections showed that the major epidemic pathogen of Ureaplasma in east of Guangdong Province is U. urealyticurn serovar 3 and serovar 6, and the distribution of different serotypes or genotypes of Ureaplasma in this area is specific.