棉花热激蛋白产生规律的初步研究

A PRIMARY STUDY ON COTTON HEAT SHOCK PROTEIN (HSP)

为探讨棉花热激蛋白（ＨＳＰ）的产生规律，选用湖北省推广的优质高产棉花（ＧｏｓｙｐｉｕｍｈｉｒｓｕｔｕｍＬ．）品种鄂棉１６和鄂抗棉３号，采用电泳、扫描等技术对棉花ＨＳＰ进行了初步研究。结果表明：热激处理可以诱导一系列ＨＳＰ的合成，同时关闭或抑制正常蛋白质的合成。ＨＳＰ诱导合成的最适条件是４２℃、２ｈ。但最适热激处理时期因品种不同而异：鄂棉１６为萌动期和一叶期，鄂抗棉３号为子叶期和一叶期。

By using fine and highyielding cotton ( Gossypium hirsutum L.) varieties, Emian 16 and Ekangmian 3 which were widely grown in Hubei province, and adopting electrophoresis, scanning techniques, a primary study on cotton HSP was conducted. The results shew: heat shock treatment induced a set of HSP sythesis, and also closed or blocked the synthesis of normal proteins. The optimium heat shock condition for inducing HSP synthesis was 42℃ for 2 hours. However, the optimium growing period for being heat shocked varied from varieties: germinating and oneleaf periods for Emian16 and cotyledon and oneleaf periods for Ekangmian 3.