旋磁场拮抗脑缺血再灌注损伤中磷脂酶A_2和内皮素作用的研究

Study of rotated magaetic field for phospholipase A2 (PLA2 ) and eadothelin ( ET ) during the blood reperfusion after cerebral ischemia

探讨旋磁场对脑缺血再灌注大鼠磷脂酶Ａ２（ＰＬＡ２）和内皮素（ＥＴ）含量的影响。方法：用四血管阻断法造成大鼠全脑缺血３０分钟后，再灌注３０分钟，此时大鼠脑组织ＰＬＡ２活性和脑组织、血浆ＥＴ的含量均显著提高，大脑皮层神经细胞和血脑屏障超微结构明显损伤。在大鼠脑缺血３０分钟后再灌注开始时，用旋磁场〔０．０８Ｔ，２５００ｒ／ｍｉｎ）作用于双侧颈总动脉区２０分钟。结果：大鼠脑组织ＰＬＡ２活性和脑组织、血浆ＥＴ含量的增高明显受抑，神经细胞和血脑屏障超微结构的损害程度减轻。结论：旋磁场有抑制ＰＬＡ２活性和降低ＥＴ水平的作用，对脑缺血再灌注损伤有一定的防治作用。

Objective , ln order to study the ,rotated magnetic field for PLA2 and ET of rats during the blood reperfusion after cerebral ischemia . Methods , The complete cerebral ischemic models were formed by fourvessels occlusion in Wistar rats After the cerebral ischemia was complete occlusion 30min and reperfusion30znin , the PLA2 activity , the ET level of brain tissue and plasma were all in- creased (P < 0. O1 ). The ultrastructure of nerve cells in cerebral cortex and blood - brain barrier was severely damaged. At the beginning of reperfusion ,the rotated magnetic field was placed on the bilateral carotid areas for 20min. Results , The PLA2 activity , the ET level of brain tissue and plasma were obvi- ously inhibited. The damag of ultrastructure of nerve cells and blood - brain barrier was slight. Conclu- sions , lt is proved that the rotated magnetic field may inhibit the PLA2 activity and reduce the ET level. lt has a prophylaxis and treatment effect for reperfusion damage after cerebral ischemia.