淡水小球藻异养培养生产叶黄素的研究

Production of lutein by the microalga Chorella ellipsoidea in heterotrophic cultivation

通过单因素实验和正交实验确定了淡水椭圆小球藻异养培养的最佳条件为BG-11培养基中葡萄糖浓度为20g/L、尿素浓度为1.0g/L、培养基初始pH值为6.5、28℃,异养培养生产叶黄素的最佳条件为BG-11培养基葡萄糖浓度40g/L、尿素浓度0.2g/L、pH值6.5、28℃下振荡培养8d。

By the single factor and orthogonal experiments, the optimum growth conditions for Cholerra ellipsoidea in heterotrophic cultivation were： BG11 medium with 20g/L glucose and 1.0g/L urea, original pH 6.5, 28℃, and lutein producing conditions were： BG11 medium with 40g/L glucose and 0.2g/L urea, original pH 6.5, 28℃, 8 days.