c-met mRNA在乳腺癌术后腋窝引流液中表达的意义

Expression of c-met mRNA in The Drainage from Axillary Fossa after Operation for Breast Cancer

目的探讨c-met mRNA在乳腺癌患者术后腋窝引流液中表达的意义。方法用RT-PCR法检测乳腺癌患者术后腋窝引流液中c-met mRNA的表达,分析其表达与肿瘤大小、腋窝淋巴结转移、雌激素受体(estrogen receptor,ER)、孕激素受体(progesterone receptor,PR)及生长因子受体2(c-erbB-2)的关系,以及5-FU加蒸馏水冲洗手术野对c-met mRNA表达的影响。结果①c-met mRNA能在乳腺癌术后腋窝引流液中表达,其阳性率高于病理检查腋窝淋巴结转移的阳性率(P<0.05)。②腋窝淋巴结转移、肿瘤大小与c-met mRNA的表达有关。③肿瘤ER、PR及c-erbB-2的表达与c-met mRNA的表达无关。④5-FU加蒸馏水冲洗手术创面能明显降低c-met mRNA的表达。结论c-met mRNA是乳腺癌微转移研究的理想特异性标志物,与常规病理检查腋窝淋巴结相比,RT-PCR检测腋窝引流液中c-met mRNA的表达能更早检测出肿瘤细胞在胸壁淋巴管中的转移,并具有更高的准确性。

Objective To study the significance of c-met mRNA in axillary drainage after operations for breast cancer. Methods RT-PCR assay was used to examine c-met mRNA in axillary drainage after operations in 52 cases of breast cancer. The relationships between the expression of c-met and the tumor size, metastatic lymph nodes, the expressions of estrogen receptor （ER）, progesterone receptor （PR） and c-erbB-2 were analyzed, respectively. In addition, the effect of douching operative field with 5-FU and distilled water on the expression of c-met mRNA was also analyzed. Results ①The proto-oncogene c-met mRNA could be detected in axillary drainage after operations for breast cancer by RT-PCR, and its positive rate was higher than that in routine pathological detection for micrometastasis in the axillary lymph nodes （P〈0.05）. ②The expression of c-met mRNA was correlated with both the metastatic lymph nodes and tumor size. ③There was no significant relationship between the expression of c-met mRNA and the expressions of ER, PR and c-erbB-2. ④Dounching operative field with 5-FU and distilled water could decrease the expression of c-met mRNA. Conclusion The proto-oncogene c-met mRNA may be an ideal and specific marker for dectecting micrometastasis of breast cancer. In addition, it also suggests that the examination of c-met mRNA in the axillary drainage by RT-PCR assay could detect the micrometastasis in axillary lymph nodes much easier and more accurately than routine pathological method.