胃癌及癌旁组织中SKP_2和p27蛋白表达及相互关系研究

Expressions of S-Phase Kinase-Associated Protein 2 and p27 Protein in Gastric Carcinoma and Pericancerous Tissues

目的研究胃癌及其癌旁组织中SKP2和p27蛋白表达水平,探讨两者的相互关系及其临床意义。方法49例胃癌及20例癌旁组织常规制作石蜡包埋切片,SKP2和p27染色方法为SP免疫组化法。结果胃癌组织中SKP2表达阳性率及其评分值明显高于癌旁组织(P<0.01),而p27则相反(P<0.05,P<0.01);SKP2表达阳性和(或)p27表达阴性的癌旁组织呈中至重度不典型增生。SKP2和p27蛋白表达与癌细胞浸润深度、有无淋巴结转移、淋巴结转移分站及有无远处转移有关(P<0.05,P<0.01)。在胃癌组织中SKP2与p27评分值之间呈负相关(r=-0.65,P<0.01)。结论SKP2和p27可能是反映胃癌发生、进展、生物学行为及预后的标记物。

Objective To study the expressions of SKP2 and p27 in gastric carcinoma and pericancerous tissues and to detect the relationship between their expressions and clinicopathological features. Methods Forty-nine eases of gastric carcinoma spicemen and 20 cases of tissue adjacent to the carcinoma were cut and made into paraffinembedded slices. The expressions of SKP2 and p27 were then detected by SP immunohistochemical method. Results The positive expression rate and score of SKP2 were both significantly higher in the gastric carcinoma tissues than those in pericancerous tissues （P〈0. 01）, whereas those terms of p27 were higher in pericancerous tissues （P〈 0.05, P〈0.01）. It was observed that the pericancerous tissues with positive SKP2 expression or with negative p27 expression showed atypical hyperplasis ranging from moderate to severe degrees. The positive rate and score of SKP2 were significantly lower in the cases of infiltrating depth T1＋T2, without-metastasis of lymph node, with-metastasis of the first site lymph node, and without-metastasis of distant organs than those in infiltrating depth T3 ＋ T4, with-metastasis of lymph node, with-metastasis of the second or third site lymph node and with-metastasis of distant organs in gastric carcinoma tissues （P〈0.05, P〈0.01 ）, whereas the results were contrary for p27 （P〈 0.01）. There was a negative correlation between the score of SKP2 and that of p27 in gastric carcinoma tissues （r=-0.65, P〈0.01）. Conclusion The expressions of SKP2 and p27 may act as important biological markers to reflect carcinogenesis, progression, biological beheviors and prognosis of gastric carcinoma.