大鼠实验性脊髓损伤后胶质瘢痕分布规律研究

The study of glial scar in the rat spinal cord after injury

目的观察慢性脊髓损伤胶质瘢痕形成的时间与空间分布特征。方法应用Allen′s法建立大鼠脊髓打击伤模型,通过神经功能评分、体感及运动诱发电位、组织病理学及免疫荧光双标记方法确定胶质瘢痕形成时间及分布。结果神经功能评分、诱发电位、组织病理学显示伤后4周进入相对稳定状态,伤后4周损伤区形成空洞,胶质瘢痕位于空洞壁。结论脊髓损伤后4周进入慢性期,研究伤后脊髓空洞及胶质瘢痕形态,为判断切除胶质瘢痕的界限提供形态学依据。

Objective To study the distribution of glial scar of spinal crod injury in rats. Methods Each rat received 150 gcf on its spinal cord at Ts with modified Allen＇s impactor. Assessment of locomotion was made with Bsaao, beattie and Bresnahan locomotor rating scale （BBB score ）, electrophysiology such as somatosensory evoked potential （SEP） and motor evoked potentials （MEP） were employed. Pathological study included hematoxylin and eosin （HE） staining, immunofluorescence double staining for glial fibrillary acidic protein （GFAP） and neurofilament （NF-200）. Results Behavioral evaluation with BBB scale and the scales was decreased to minimal 1 day postinjury, and improved thereafter. At up to 4 weeks, the scales improved to the best scales and inclined plane. The latent period of motor evoked potential （MEP） and somatosensory evoked potential （SEP） was postponed and entered flat roof by 4 weeks after injury. The glial scar and ＇cyst wen established at the injury site in 4 to 8 weeks thought to be one of the important impediments tO axonal regeneration after spinal cord injury. The the cyst wall were made up mainly of astrocytes and their processes. Conclusion It suggested that the chronic spinal cord injury began at the fourth week after injury, and at this time it is may be the opportunity for repairing. The distribution of glial scar provides experimental date for judgment of the site and extension to remove the glial scar.