鲍氏不动杆菌耐药性与ADC β-内酰胺酶研究

Resistance of Acinetobacter baumannii and ADC β-Lactmases

目的了解鲍氏不动杆菌的耐药性及其与ADCβ-内酰胺酶的关系。方法用K-B法检测115株鲍氏不动杆菌的药敏结果;分别对30株鲍氏不动杆菌用三维试验检测ADCβ-内酰胺酶、用PCR方法检测blaADC基因、用氯唑西林抑制酶试验检测头孢他啶等MIC值。结果亚胺培南、头孢哌酮/舒巴坦耐药率<10.4%,而其他抗菌药物的耐药率均>54.0%;多重耐药菌占59.0%;多重耐药菌的三维试验、blaADC基因均为阳性,除头孢吡肟外,加入氯唑西林(500 mg/L)可以使头孢他啶等抗菌药物的MIC有>8倍的降低;非多重耐药菌均为阴性。结论鲍氏不动杆菌耐药现象严重,产ADCβ-内酰胺酶菌株与多重耐药有直接关系。

OBJECTIVE To study the resistance of Acinetobacter baumannii in our hospital and the relation with ADC β-lactmases. METHODS Antibiotic susceptibility testing was determined by K-B methods to 115 A. baumannil strains, and three dimensional extract testing was performed to detect the ADC β-lactmases-producing strains, blaADC gene was detected by PCR methods, and the MIC of ceftazidime, et al were determined in the presence of cloxacillin （500 mg/L） in 30 A. baumannii strains detected. RESULTS The resistance to imipenem and cefoperazone/sulbactam was below 10. 4%, to other antibiotic resistance was over 54%; the multiresistance strains were 59%. ADC β-lactmases and blaADC genes were all positive and the MIC of ceftazidime, et al except cefepime was decreased over eight-fold in the presence of cloxacillin for multiresistant A. baumannii, but not for non-multiresistant A. baumannii. CONCLUSIONS The resistance of A. baumannil is very serious in our hospital, ADC β-1actmases-producing strains are related markedly with the multiresistance of A. baurnannii.