摘要
目的:观察冬凌草甲素对人鼻咽癌细胞株CNE-2细胞的放射增敏作用,并探讨其可能机制。方法:用克隆形成实验计算药物的放射增敏比,流式细胞仪法分别检测、比较单纯药物组、单纯照射组、联合治疗组各组的细胞周期变化。结果:冬凌草甲素的放射增敏比为1.227,流式细胞仪法分析显示,联合治疗组的细胞周期变化以G2/M期阻滞为主(45.2%),与单纯药物组、单纯照射组比较差异均有统计学意义(P<0.05)。结论:冬凌草甲素对人鼻咽癌细胞株CNE-2细胞具有一定的放射增敏作用,其机制可能与改变细胞周期有关。
Objective: To observe the radio-sensitization of Oridonin and its mechanism on nasopharyngeal carcinoma cell line CNE-2. Methods: The radiation sensitization enhancement ratio(SER) was determined by clonogenic assay. Cell cycle distribution was assessed by flow cytometry. Results: The SER was 1.227. The rate of G2/M phase block related to the Oridonin group, the radiotherapy group and the combination treatment group was 17.8 %, 24.7 % and 45.2%, respectively. Conclusion: Oridonin can enhance radiosensitition for CNE-2 cells, which maybe relate a to changes of the cell cycle.
出处
《山东大学耳鼻喉眼学报》
CAS
2007年第2期127-129,共3页
Journal of Otolaryngology and Ophthalmology of Shandong University
基金
江苏省教育厅自然科学研究计划项目(04KJD320120)
关键词
冬凌草甲素
鼻咽肿瘤
放射增敏
Oridonin
Nasopharyngeal carcinoma
Radiosensitization