喉癌组织中核转录因子NF-kB的定量检测

A quantitative analysis of NF-kB in laryngeal carcinoma

目的:探讨核转录因子(nuclear transcription factor kappa B,NF-kB)的定量检测在喉癌组织中的意义。方法:采用蛋白质印迹杂交法(Western blot法)和凝胶电泳迁移率(EMSA)法对20例喉癌组织和10例癌旁正常组织中NF-kB的蛋白含量以及NF-kB DNA结合活性进行定量检测,并进行分析比较。结果:喉癌组织中NF-kB的蛋白含量以及NF-kB DNA结合活性均明显高于癌旁正常组织,差异有统计学意义(P<0.05)。结论:NF-kB在喉癌的发生发展过程中可能起一定的作用,并为喉癌的治疗能提供一个新靶点。

Objective： To explore the significance of the quantitative analysis of NF-κB in laryngeal carcino- ma tissues. Methods： The quantitative analysis of the protein content and the DNA binding activity of NF-κB was examined by the Western blot method and electrophoretic mobility shift assay （EMSA） method in 20 cases of laryngeal carcinoma tissues and 10 cases of normal pericarcinoma tissues. Results： The protein content and the DNA binding activity of NF-κB in laryngeal carcinoma tissues were higher than those in pericarcinoma normal tissues （ P 〈 0.05）. Condusion： The NF-κB might play an important role in the occurrence and development of laryngeal carcinoma and offers a new target of laryngeal carcinoma treatment.