中药红花对白血病抑制因子在小鼠早孕子宫内膜表达的影响

Role of the Chinese drug saffron on the expression of leukemia inhibitory factor in the endometrium of early pregnant mice

目的:探讨中药红花抗早孕作用与白血病抑制因子(leukemia inhibitory factor,LIF)在小鼠子宫内膜表达的关系。方法:受精后第2天,分别给孕鼠灌服不同剂量的红花浸液(10.5 g/kg、15.5 g/kg和20.5 g/kg)和生理盐水,连续3天。停药后24 h取子宫,石蜡包埋制片,进行HE和LIF免疫组化染色;将免疫组化染色结果输入图像分析仪,对LIF蛋白的表达进行定量分析。结果:各实验组孕鼠子宫内膜充血,上皮细胞排列紊乱,且随着药物剂量的提高逐渐改变。在对照组,孕鼠子宫内膜LIF呈强阳性表达,在实验组的表达水平显著低于对照组(P<0.05,P<0.01);随着红花剂量的提高,LIF的表达逐渐减少。结论:中药红花可抑制孕鼠子宫内膜细胞LIF蛋白的表达,从而影响胚胎的着床。

Objective： To explore the relationship between the saffron and the expression of leukemia inhibitory factor（LIF） in the endometrium of early pregnant mice. Methods： The mated female mice were orally treated with saffron solution in a dose of 10.5 g/kg, 15.5 g/kg and 20.5 g/kg or physiological saline （control）, on the 2nd, 3rd and 4th days after fertilization. The uteri of the mice were removed and routinely sectioned 24 hours later. The morph of the endometrium was observed with HE and the expression of LIF in the endometrium of the mice was analyzed with immuno-histochemistry and Quantimet. Results： The endometrium was in congestion and disorder in the experimental groups; with increasing doses of saffron, the endometrium gradually changed. The LIF was expressed strongly in the murine endometrium of control group. The expression level of LIF in experimental groups was much lower than that in the control group（ P 〈0.05, P 〈0.01）and decreased gradually with increasing doses of saffron. Conclusion： Saffron can inhibit the expression of LIF in the endometrium of pregnant mice, and therefore interferes with the implantation of embryos.