Ⅱ型糖尿病患者房水中VEGF和IL-6含量的检测

Level of VEGF and IL-6 in the aqueous humor of Ⅱ diabetic patients

目的:检测Ⅱ型糖尿病患者眼房水中血管内皮生长因子(vascular endothelial growth factor,VEGF)和白细胞介素-6(interleukin-6,IL-6)的含量,并探讨其临床意义。方法:采用双抗体夹心酶联免疫吸附(ELISA)法测定64例Ⅱ型糖尿病患者房水中VEGF和IL-6的含量,根据散瞳眼底检查和眼底荧光素血管造影检查后,实验组分为:无糖尿病视网膜病变组(NDR)21例、单纯型糖尿病性视网膜病变组(BDR)25例、增生型糖尿病性视网膜病变组(PDR)18例,对照组为健康的老年性白内障患者20例。结果:NDR组、BDR组、PDR组的房水VEGF含量分别为(220.27±25.69)pg/ml(、284.31±58.92)pg/ml(、459.07±90.90)pg/ml,IL-6含量分别为(154.09±32.76)pg/ml(、221.81±60.52)pg/ml(、387.95±90.22)pg/ml,对照组房水VEGF含量为(135.50±25.69)pg/mlI、L-6含量为(78.71±20.37)pg/ml,对照组与实验组比较差异均有统计学意义(F=108.72,P<0.01;F=102.532,P<0.01)。4组房水VEGF和IL-6含量有明显增加的趋势。房水中的VEGF含量与相应的IL-6含量有相关性(r=0.995,P<0.01);房水中VEGFI、L-6含量与糖尿病患者的病程及糖尿病视网膜病变的严重程度有相关性(r=0.722,P<0.01;r=0.716,P<0.01)、(r=0.869,P<0.01;r=0.865,P<0.01)。结论:VEGFI、L-6在糖尿病视网膜病变的形成过程中有重要作用,且两者之间有相关性。

Objective： To explore the role of VEGF and IL-6 in the aqueous humor in the pathogenesis of diabetic retinopathy （DR） . Methods： Aqueous humor samples were collected from diabetic patients with cataracts or without retinopathy, and aqueous humor samples from non-diabetic patients with cataracts were chosen as controls. The content of VEGF in the aqueous humor was determined by using the ELISA technique. Results： The level of VEGF in the aqueous humor samples of diabetic patients without retinopathy（NDR）, background diabetic retinopathy （BDR） and proliferative diabetic retinopathy （PDR） were（220.27 ± 25.69）pg/ml, （284.31 ± 58.92）pg/ml and （459.07 ± 90.90）pg/ ml. The level of IL-6 in the aqueous humor from NDR, BDR and PDR were（ 154.09 ± 32.76）pg/ml, （ 221.81 ±60.52 ）pg/ml and （387.95 ± 90.22）pg/ml. The levels of VEGF and IL-6 in the aqueous humour from the control group were （135.50 ± 25.69）pg/ml, （ 78.71 ± 20.37）pg/ml. Compared with the control, the level of VEGF and IL-6 in the aqueous humor of diabetic patients increased significantly （ P 〈 0.01 ）. The level of VEGF and IL-6 in the aqueous of the NDR, BDR and PDR also increased significantly （ P 〈 0.01 ）. The level of VEGF significantly correlated with the level of IL-6 in the aqueous humor（ r = 0.995, P 〈 0.01 ）. The level of VEGF and IL-6 in the aqueous humor also significantly correlated with the severity and prolongation of DR（ r = 0.869, P 〈 0.01 ; r = 0.865, P 〈 0.01）. Conclusion：VEGF and IL-6 play active roles in the generation and development of DR, and the level of VEGF in the aqueous humor closely correlates with the level of IL-6 in the aqueous humour of diabetic patients.