子宫内膜癌患者FHITS、LIT2、EDNRB基因杂合性丢失

Heterozygosity loss at FHIT,SLIT2 and EDNRB gene in endometrical carcinoma

目的:探讨子宫内膜癌患者FHIT、SLIT2、EDNRB基因3个微卫星位点D3S1287、D4S1593、D13S160杂合性丢失(loss of heterozygosity,LOH),以确定侯选的抑癌基因。方法:应用PCR-变性PAGE-银染方法分别对35例子宫内膜癌患者癌组织及相对应的正常子宫内膜组织在FHIT、SLIT2、EDNRB基因3个微卫星位点D3S1287、D4S1593、D13S160行LOH检测。结果:LOH总检出率为54.3%,D3S1287、D4S1593、D13S160位点分别为34.5%、20.5%、19.3%。FHIT、SLIT2、EDNRB基因的3个微卫星位点发生LOH率与子宫内膜癌手术-病理分期无明显相关性。结论:子宫内膜癌患者肿瘤组织在FHIT、SLIT2、及EDNRB基因的微卫星位点D3S1287、D4S1593、D13S160均有LOH。FHITS、LIT2及EDNRB基因为抑癌基因,其失活可能与子宫内膜癌的发生有关。

Objective： To study the loss of heterozygosity（LOH） at FHIT, SLIF2 and EDNRB gene at microsatellite markers D3S1287, IMS1593, and D13S160 in endometrial carcinoma. Methods： Tumor tissues and relatively normal tissues of thirty-five cases of endometrial carcinoma were examined by using PCR-denaturing PAGE-silver staining at microsatellite markers D3S1287, D4S1593, and D13S160. Results： The total LOH rate was 54.3%, and LOH rate of D3S1287, D4S1593, and D13S160 was 34.5%, 20.5%, and 19.3%, respectively. The LOH rate at FHIT, SLIT2, and EDNRB gene did not positively correlate with the stage of clinical-pathogensis in endometrial carcinoma. Conclusion： LOH exists at micro-satellite markers of FHIT, SLIT2, and EDNRB gene in endometrial carcinoma, suggesting that FHIT, SLIT2, and EDNRB gene are tumor suppressor genes（TSGs） and the inactivity of TSGs maybe one of the reasons for carcinoma.