葡萄子原花青素对糖尿病大鼠心肌超微结构的影响

Changes of myocardial ultrastructure induced by grape seed proanthocyanidin extracts in STZ diabetic rats

目的:观察葡萄子原花青素(grape seed proanthocyanidin extracts,GSPE)对链脲佐菌素(streptozoto-cin,STZ)糖尿病大鼠血浆糖基化终末产物(advanced glycation end products,AGEs)、心肌超微结构的影响;探讨GSPE对STZ糖尿病大鼠心肌的保护作用。方法:雄性Wistar大鼠尾静脉注射0.1%STZ以建立糖尿病模型,成模后随机分为2组,糖尿病对照组(DM1组,n=8)和糖尿病GSPE治疗组(GSPE250 mg/(kg.d),DM2组,n=12),另设2组,正常对照组(C1组,n=10)和正常GSPE治疗组(C2组,n=10)。24周后采血测血糖、糖化血红蛋白(glycosylated hemoglobin,HbA1c)、AGEs,电镜观察心肌超微结构变化。结果:DM2组AGEs较DM1组明显降低(t=2.792,P=0.02);DM1组肌原纤维和线粒体排列紊乱,经GSPE治疗后DM2组明显减轻。结论:GSPE能够抑制STZ糖尿病大鼠非酶糖基化反应,对其心脏超微结构有一定保护作用。

Objective： To observe the effect of grape seed proanthocyanidin extracts （GSPE）on plasma advanced glycation end products （AGEs） and myocardial ultrastructures of streptozotocin （STZ） induced diabetic rats. Methods： Male Wistar rats were subjected to 0.1% STZ through the tail vein to induce diabetics. Then, they were randomly divided into two groups： the diabetic model （DM） group （DM1, n = 8）and the GSPE-treatment diabetic group （DM2, n = 12, 250 mg·kg^-1·d^-1）. Non-diabetic Wistar rats were enrolled in an other two groups： the control group （C1, n = 10） and the GSPE -treatment group（C2 group, n = 10, 250 mg·kg^-1·d^-1 ）. After 24 weeks, all rats were killed, and the fasting plasma glucose, glycosylated hemoglobin and AGEs were determined. Changes of myocardial ultrastructure were observed by an electronic microscope. Results： Plasma AGEs of DM2 group was lower than that of DM1 group （ t = 2.792, P = 0.02）. The myofibrillaes and the chondriosomees were disordered in DM1 group, but changed after the GSPE treatment. Conclusion： GSPE can inhibit nonenzymatic reactions of glycosylation in STZ-induced diabetic rats and protect the myocardial ultra-structure.