福氏痢疾杆菌感染后不同治疗方法对大鼠肠功能的影响

Effects of various treatments on bowel function after shigella flexneri infection in rats

目的:观察福氏痢疾杆菌感染后大鼠肠道感觉运动功能的改变及不同治疗方法的作用,探讨预防急性肠道感染后肠功能紊乱的方法,从而为感染后肠易激综合征(PI-IBS)的预防提供指导。方法:将40只雌性Wistar大鼠分为正常对照组、模型组、抗生素治疗组、微生态制剂治疗组和抗生素与微生态制剂联合治疗组5组。后4组应用福氏痢疾杆菌造成肠道感染后,分别行相应治疗。在感染后16-22 d,所有大鼠进行直肠气囊扩张,测定肠道感觉阈值和离体肠道平滑肌的肌张力,并行远端结肠病理学检查。观察肠道感觉运动功能的改变和组织学改变。结果:各组大鼠组织学均无明显变化;模型组、抗生素治疗组和微生态制剂治疗组的肠道感觉阈值较正常组明显降低(P<0.05),离体平滑肌收缩的张力积分明显增高(P<0.05);联合治疗组肠道感觉阈值及离体平滑肌收缩的张力积分与正常组无明显差异。结论:①大鼠在福氏痢疾杆菌感染炎症消失后还存在着肠功能紊乱,表现为肠道感觉运动功能的异常;②单纯应用抗生素或微生态制剂治疗,无法使肠道的感觉运动功能恢复正常;③联合应用抗生素和微生态制剂治疗,可以使肠道感觉运动功能恢复正常,从而预防急性肠道感染后肠功能紊乱的发生。

Objective： To observe the changes of bowel sensory-motor function after shigella flexneri infection and the effects of various treatment for bowel function in rats, and therefore to provide guidance for preventing PI-IBS. Methods： Forty female Wistar rats weighted 170 - 180 g were divided into normal control and model, antibiotics-treatment, probiotics-treatment and combination treatment groups. The 32 rats in the latter 4 groups were first infected with shigeUa flexneri by gavage to establish the model of acute infectious enteritis, and the normal controls were treated with 0.9% saline. Second at 16- 22 days after bowel inflammation, the perceptual threshold was detected and the contraction of enteric smooth muscle was measured in vitro to observe the changes of bowel sensitivity and motility and at the same time histological changes were observed. Results： No obvious histological changes were found in different groups. The perceptual threshold decreased and the tension integral of enteric smooth muscle greatly increased in the model group, antibiotics-treatment and probiotics-treatment groups （ P 〈 0.05）, while that of the combination treatment group had no difference from the normal control. Conclusion： Shigella flexneri infection can lead to bowel sensory-motor disorder that remains for a period of time after inflammation diminishes. Treatment with antibiotics or probiotics singlely cannot normalize the functional disturbance, but a combination treatment can.