1Liang MF,Dubel S.Production recombinant human IgG antibodies in the baculovirus expression system[M].Antibody Engineering.Springer-verlay,Berlin,Heidellery New York,2001,334-356.
2Yahata T,Andriole S,Isselbacher KJ,et al.Estimation of baculovirus titer by β-galactosidase activity assay of virus preparations[J].BioTechniques 2000,29(2):214-215.
3Sussman DJ.24-Hour assay for estimating the titer of β-galactosidase-expressing baculo virus[J].BioTechniques 1995,18:50-51.
4Cha HJ,Gotoh T,Bentley WE.Simplification of titer determination for recombinant baculovirus by green fluorescent protein marker[J].BioTchniques 1997,23(5):782-786.
5Lo HR & Chao TC.Rapid titer determination of baculovirus by quantitative real-time polymerase chain reaction[J].Biotechnol Prog 2004,20:354-360.
7Kwon MS,Dojima T,Toriyama M,et al.Development of on antibody-based assay for determination of baculovirus titers in 10 hours[J].Biotechnol Prog 2002,18:647-651.
9Bac PAK TM Baculovirus rapid titer kit[EB].http://www.clontech.com.
10Philipps B,Forstner M,Mayr LM,et al.Baculovirus expression system for magnetic sorting of infected cells and enhanced titer determination[J].BioTechniques 2004,26 (1):80-83.
二级参考文献9
1Huser A, Hofmann C. Baculovirus vectors: novel mammalian cell gene-delivery vehicles and their applications [J]. Am J Pharmacogenomics,2003;3(1):53-63.
2Tani H, Limn C K, Yap C C et al. In vitro and in vivo gene delivery by recombinant baculoviruses [J]. J Virol,2003;77(18):9799-9808.
3Smith A E. Viral vectors in gene therapy [J]. Annu Rev Microbiol,1995;49:807-838.
4Saldanha J. Validation and standardization of nucleic acid amplification technology (NAT) assays for the detection of viral contamination of blood and blood products [J]. J Clin Virol,2001;20(1-2):7-13.
5Wilhelm J, Pingoud A. Real-time polymerase chain reaction [J]. Chembiochem,2003;74(11):1120-1128.
6Zen Z, Espinoza Y, Bleu T et al. Infectious titer assay for adeno-associated virus vectors with sensitivity sufficient to detect single infectious events [J]. Hum Gene Ther,2004;15(7):709-715.
7Tsang R K, Vlantis A C, Ho R W et al. Sensitivity and specificity of Epstein-Barr virus IGA titer in the diagnosis of nasopharyngeal carcinoma: a three-year institutional review [J]. Head Neck,2004;26(7):598-602.
8Sambrook J, Fritsch E F, Maniatis T.Molecular cloning.In:A laboratory manual [M].2nd ed.New York:Cold Spring Harbor Laboratory Press,1989:16-69.
