白藜芦醇对麻醉大鼠颈动脉窦压力感受器的作用(英文)

Effect of resveratrol on baroreceptor activity of carotid sinus in anesthetized male rats

在隔离灌流左侧颈动脉窦区的麻醉大鼠上观察了白藜芦醇对颈动脉窦压力感受器活动的影响。隔离灌流麻醉大鼠的颈动脉窦区,同时记录窦神经放电,并绘制压力感受器活动的机能曲线。白藜芦醇(30,60及120μmol.L-1)隔离灌流颈动脉窦区时,压力感受器活动的机能曲线向右下方移位,曲线的斜率以及窦神经放电的最大积分值显著下降,且其变化呈一定的剂量依赖性。预先应用NO合酶抑制剂(L-NAME,100μmol.L-1)可完全消除白藜芦醇对压力感受器活动的抑制作用;预先应用钙通道的开放剂(Bay K8644,500 nmol.L-1)可以取消白藜芦醇的抑制作用;预先应用正矾酸钠(sodium orthovanadate,1 mmol.L-1)后,对白藜芦醇抑制压力感受器活动的作用无影响。白藜芦醇对大鼠颈动脉窦压力感受器活动有抑制作用,此作用可能与局部NO的释放及减弱牵张敏感性通道介导的钙离子内流有关。

This study is to evaluate the effect of resveratrol on carotid baroreceptor activity （CBA）. The functional curve of carotid baroreceptor （FCCB） was constructed and the functional parameters of carotid baroreceptor were measured by recording sinus nerve afferent discharge in anesthetized male rats with perfused isolated carotid sinus. Resveratrol （30, 60 and 120μmol · L^-1） inhibited CBA, which shifted FCCB to the right and downward. There was a marked decrease in peak slope （PS） and peak integral value （PIV） of carotid sinus nerve charge in a concentration-dependent manner. Pretreatment with N^w-nitro-L-arginine methyl ester （L-NAME, 100μmol · L^-1）, an inhibitor of nitric oxide synthase （NOS）, eliminated the inhibitory effect of resveratrol. Pretreatment with Bay K8644 （an agonist of L-type calcium channel, 500mmol · L^-1 ） abolished the effect of resveratrol on CBA. A potent inhibitor of tyrosine phosphatase （sodium orthovanadate, 1 mmol · L^-1 ） did not influence the effect of resveratrol on CBA. Resveratrol inhibits carotid baroreceptor activity, which may be mediated by the locally released NO and decreased calcium influx. Several studies have showed a cardioprotective effect of resveratrol, with the penetrating study of resveratrol, it may show a potential value in the clinical treatment of cardiovascular disease as an alternative medicine.