重型颅脑损伤后大鼠胃肠动力障碍与血浆MTL、VIP变化的实验研究

An experimental study on disturbance of gastrointestinal motility and change of motilin,vasoactive intestinal peptide in plasma in rats with severe head injury

目的研究重型颅脑损伤大鼠胃肠动力和血浆胃动素(MTL)和血管活性肠肽(VIP)的改变,探讨重型颅脑损伤后胃肠动力障碍发生的相关因素。方法雄性Wistar大鼠64只,随机分为假手术组、重型颅脑损伤组,每组分为伤后6、24、48、72h组,每组8只。每组测定胃排空、肠道传输速率及MTL和VIP含量。结果大鼠重型颅脑损伤后6、24、48、72h胃排空和肠道传输速率显著减弱,血浆MTL含量显著升高,血浆VIP含量在伤后6h变化差异无统计学意义,24、48h显著下降,至伤后72h逐渐恢复。结论大鼠重型颅脑损伤后在伤后6h已出现胃肠动力障碍,可持续72h以上,与血浆中MLT和VIP变化相关。

Objective To study the change of gastrointestinal motility and motilin （MTL） ,vasoactive intestinal peptide（VIP） in plasma in rats with severe head injury （SHI） and to investigate the factors of gastrointestinal motility disturbance following SHI. Methods Sixty-four male Wistar rats were randomly divided into sham operated groups and SHI groups at 6,24,48,72h（8 rats each group ） . The gastric emptying,intestinal transmission rate and content of MTL,VIP in plasma were detected. Results The gastric emptying,intestinal transmission rate were significantly weakened, and the content of MTL in plasma was significantly heightened in rats at 6,24,48,72h following SHI. The content of VIP in plasma had no statistical change at 6h, but it descended significantly at 24,48h and heightened gradually at 72h. Conclusion The gastrointestinal motility disturbance were shown at 6h and lasted 72h in rats following SHI, it correlated to the content of MTL and VIP in plasma.