针刺预处理对全脑缺血大鼠微血管损伤及脑水肿的保护作用

Protection of Acupuncture Preconditioning Against Microvascular Injury and Brain Edema in Global Ischemic Rats

[目的]针刺预处理对全脑缺血大鼠微血管损伤及脑水肿的保护作用.[方法]采用四动脉阻断法制作大鼠全脑缺血模型,针刺预处理组:术前给予针刺,针刺7 d后给予全脑缺血10 min,干湿法测脑组织水含量,应用HE染色、TUNEL染色及透射电镜技术,观察大鼠脑缺血后,组织病理学及超微结构改变.[结果]与脑缺血组相比,针刺预处理组不引起明显的神经元损伤,全脑缺血10 m in后72 h,微血管损伤及脑水肿明显减轻,血管内皮细胞凋亡减少.[结论]针刺预处理能够通过减轻微血管损伤及脑水肿对脑缺血产生保护作用.

[ Objective ] To explore the protection of acupuncture preconditioning against microvascular injury and brain edema in global ischewic rats. [ Methods]To prepare global ischewic model by four-vessel occlusion method. Seven days before 10 win global ischewia,the rats received acupuncture in acupuncture preconditioning group. The water content were evaluated by dry-wet method. The pathohistology and ultrastructure were observed by HE staining, TUNEL staining and transmission electron microscopy. [ Results ] To compare brain ischemic group with acupuncture preconditioning group, wicrovascular injury and brain edema relieved obviously and vascular endothelial cell apotosis decreased in the later. [ Conclusion ] Acupuncture preconditioning can protect against brain ischemia by reducing wicrovascular injury and brain edema.