摘要
目的探讨高间接胆红素血症新生儿外周血单个核细胞(PBMC)凋亡率与血浆8-异-前列腺素F2α(8-iso-PGF2α)水平的相关性,证实高胆红素血症所致的脂质过氧化损伤。方法日龄2～7d新生儿129例分为4组:轻度黄疸组43例,中度黄疸组38例,重度黄疸组18例和健康对照组30例。应用酶免疫分析法(EIA)测定其血浆8-iso-PGF2α水平,流式细胞仪检测其PBMC凋亡率。结果1.健康对照组与轻度黄疸组PBMC凋亡率无明显差异(P=0.108);随黄疸程度加重,患儿PBMC凋亡率升高;2.健康对照组血浆8-iso-PGF2α水平[(14.74±6.71)ng/L)]与轻度黄疸组[(15.79±5.76)ng/L]比较无明显差异(P=0.502);中、重度黄疸组血浆8-iso-PGF2α水平升高(Pa=0);3.PBMC凋亡率与血浆8-iso-PGF2α水平存在正相关(r=0.602P=0)。结论高胆红素血症可引起新生儿脂质过氧化损伤,PBMC凋亡增加。血浆8-iso-PGF2α水平是反映脂质过氧化损伤新生儿的一项可靠生化指标。
Objective To explore the correlation of the apoptesis of peripheral blood mononuclear cell （PBMC） With plasma 8 - iso - prostaglandin F2α （8 - iso - PGF2α ） level in order to confirm the perexidation damage of hyperbilimbinemia in neonates With jaundice. Methods One hundred and twenty - nine neonates who were 2 to 7 days old were divided into 4 groups： slight jaundice （ 43 cases）, moderate jaundice （38 cases）, serious jaundice （ 18 cases） ,and healthy control（30 cases） groups. Plasma 8 - iso - PGF2α levels were assayed by enzyme immunoassay（ EIA）, and the apoptosis rates of PBMC were determined by flow cytometry. Results 1. There was no significant difference in the apoptesis rate of PBMC between normal and slight jaundice neonates （P = 0. 108 ） ; apoptosis rate of PBMC was increasing With the aggravation of jaundice. 2.Plasma 8 -iso- PGF2α level in normal neonates was 14.74 ± 6.71 ng/L, there was no difference between normal neonates and neonates with slight jaundice（ P = 0. 502 ）. Plasma 8 - iso - PGF2α levels in neonates with moderate and serious jaundice were much higher （ P1 = 0）. 3. Positive correlation existed between plasma 8 - iso - PGF2α level and PBMC apoptesis rate （ r = 0. 602 P = 0 ）. Conclusions Hyperbilirubinemia can induce perexidation damage,resulting in increase of PBMC apoptosis. Plasma 8 -iso -PGF2α level can accurately evaluate the peroxidation damage in neonates With jaundice.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2007年第10期762-763,800,共3页
Journal of Applied Clinical Pediatrics
