流动注射化学发光法测定二胺氧化酶

Flow-injection chemiluminescence system for the determination of diamine oxidase

二胺氧化酶是具有高度活性的细胞内酶，能催化二胺类物质的分解．基于Lumlnol-Co^2＋ -H2O2化学发光体系，将酶促反应与化学发光反应相耦合，并结合流动注射技术，通过检测H2O2的发光信号，间接测定二胺氧化酶的活性．该方法操作简单、快速、灵敏度高、重现性好．二胺氧化酶在1×10^-3 ～6×10^-2 U／L活性范围内与化学发光强度具有良好的线性关系，检出限为3．0×10^4 U／L，RSD为6．7％．

Diamine oxidase is a kind of intracellular enzyme which possesses high activity. It can catalyze diamines to produce hydrogen peroxide （H2O2）. Based on the chemiluminescence system of Luminol-Co^2＋ -H2O2, and combined with flow-injection technique, a chemiluminescence method for the determination of diamine oxidase was developed. The present method has following advantages：simplicity, rapid analysis, high reproducibility and sensitivity. The linear range is 1×10^-3 ～6×10^-2 U/L, the relative standard deviation （RSD） is 6.7% for 1× 10^-3 U/L of DAO with a detection limit of 3.0×10^4 U/L. Key words, flow-injection; chemiluminescence; diamine oxidase; cadaxerine