氟喹诺酮类药物体外多步诱导人型支原体耐药的研究

Study on the drug resistance of Mycoplasma hominis in vitro multiple step induced by fluoroquinolones

目的探讨氟喹诺酮类药物体外多步诱导人型支原体耐药的状况,指导临床合理用药。方法将Mh标准株和临床分离敏感株在体外进行四种氟喹诺酮类药物的多步诱导,然后检测其诱导前后对这四种药物的MIC变化。结果所有Mh敏感株经四种药物诱导后对诱导药物和非诱导药物均产生不同程度的耐药和交叉耐药,且这种体外获得性耐药具有较好的稳定性。结论体外长时间、低浓度的氟喹诺酮类药物刺激将诱导Mh产生耐药和交叉耐药,这对临床合理选用抗生素具有重要的指导意义。

Objective To explore the resistance of Mycoplasma hominis to fluoroquinolones in vitro for guiding reasonablly chaical apphcatlon of antibiotics. Methods A Mycoplasma hominis standard strain and two clinical strains were induced stepwise with four kinds of fluoroquinolones, and minimum inhibition concentrations were detected before and after inducement. Results .All Mh sensitive strains became resistant and cross - resistant to four kinds of inducing and non - inducing fluoroquinolones after induced. Conclusion The resistance and crossresistance of Mycoplasma hominis to fluoroquinolones can be induced after In vito long time and lower concentration stimulation with the fluoroquinolones, it helpful for guidance of reasonable choice of antibiotics in clinical practice.