槐耳浸膏对骨髓瘤细胞增殖和凋亡的影响

Impact of Huaierjingao on the proliferation and apoptosis of myoloma cell induced by PS-T

目的探讨槐耳浸膏对骨髓瘤细胞株PRMI8226细胞增殖和凋亡的影响。方法取PRMI8226细胞与1.5mg/ml、3mg/ml、5mg/ml的不同浓度槐耳浸膏在体外孵育,分别在24h、36h、和48h时用CCK-8法检测细胞增殖抑制率,并用流式细胞术(FCM)检测早期细胞凋亡情况。结果槐耳浸膏可抑制PRMI8226细胞增殖,不同浓度的槐耳浸膏对多发性骨髓瘤细胞增殖的抑制效果不同,以5mg/ml抑制增殖效果最好。且浓度为5.0mg/ml的金克诱导多发性骨髓瘤细胞48h抑制率达到高峰84%。FCM检测表明槐耳浸膏可诱导PRMI8226细胞早期凋亡,用5.0mg/ml槐耳浸膏处理48h后,可引起25.9%的PRMI8226细胞凋亡。这两种作用均随槐耳浸膏浓度和作用时间的延长而增强。结论槐耳浸膏可在体外抑制PRMI8226细胞增殖,并促进其凋亡。

Objective To investigate the effects of Huaierjingao （PS - T） on proliferation and apeptosis of myelorna cell llne PRMI8226 in vitro. Methods PRMI8226 eells were cultured with different concentrations of PS - T （1.5, 3.0 and 5. Omg/ml）. The inhibition of proliferation was measured by CCK - 8 assay 24, 36 and 48 hours after culture and cell apeptosis was evaluated by flow eytometry. Results PS - T inhibited the growth of PRMI8226 cells in concentration and time - dependent manner. Flow eytometry analysis showed that PS - T induced apeptosis of PRMI8226 cells, and the percentage of apoptotie cells increased as the concentration increased and time elongated. Conclusion PS - T can inhibit proliferation of PRMI8226 cells and promote its apeptosis.