酸敏感离子通道在唾液腺腺样囊性癌中的表达

Expression of acid-sensing ion channels in adenoid cystic carcinoma of salivary glands

目的:检测酸敏感离子通道(acid-sensing ion channels,ASICs)在唾液腺腺样囊性癌(ACC)中的表达情况,并探讨ASICs与ACC侵袭转移的关系。方法:采用免疫印迹和免疫组织化学方法,检测ASICs在培养的ACC-2细胞及腺样囊性癌组织中的表达。采用方差分析,利用OriginPro7.0软件进行统计学分析。结果:ACC-2细胞和腺样囊性癌组织中有ASICs蛋白的表达,而在正常组织中未见ASIC2a的表达;ASICs蛋白表达量经灰度值分析,正常腮腺组织为0.00012±0.00044,多形性腺瘤组织为0.00023±0.00049,腺样囊性癌组织为0.57±0.00045,差异有显著性意义,P<0.001。结论:腺样囊性癌中ASICs表达增多,可能是腺样囊性癌侵袭转移的组织病理学基础。

PURPOSE： To investigate the expression of acid-sensing ion channels （ASICs） in adenoid cystic carcinoma （ACC） of salivary glands, and evaluate the role of ASICs in the invasion of ACC. METHODS： Expression of ASICs in cultured ACC-2 cells and samples from normal salivary glands（NA） ,pleomorphic adenoma （PA） tissues or ACC tissues was detected by western blotting and immunohistochemistry. Statistical analysis was performed by one-way analysis of variance （ANOVA） with OriginPro software （Version 7.0）. RESULTS： The enhanced expression of ASICs was observed in cultured ACC-2 cells and ACC tissues. Moreover, the enhanced ASIC2a expression was observed in the nuclei of ACC. （NA： 0.00012±0.00044, PA： 0.00023±0.00049,ACC： 0.57±0.00045, P〈0.001）. CONCLUSION： The enhanced density of ASICs expression in ACC may contribute to the invasion of ACC.