胡黄连苷Ⅱ在谷氨酸诱导的PC12细胞损伤中的保护作用

Protective effects of picrosideⅡon glutamate injury of PC12 cells

目的:以谷氨酸为工具药建立氧化应激模型,观察胡黄连苷Ⅱ的保护作用并初步探讨其作用机制。方法:分别用不同浓度的胡黄连苷Ⅱ和10mmol/L的谷氨酸处理PC12细胞,通过MTT法检测细胞的活力,CDCFH染色法检测细胞氧自由基水平,DNA琼脂糖凝胶电泳检测细胞凋亡,Western blot检测bcl-2表达。结果:胡黄连苷Ⅱ能明显减轻谷氨酸对PC12细胞的损伤,提高细胞的存活率,降低细胞内的氧自由基水平,上调Bcl-2蛋白的表达,抑制细胞凋亡。结论:胡黄连苷Ⅱ对谷氨酸引起的PC12细胞损伤有保护作用,其作用机制可能与抑制或清除谷氨酸诱导的细胞内活性氧的生成,调节凋亡相关基因Bcl-2蛋白的表达有关。

AIM： To investigate the effect of picroside Ⅱ on apoptosis induced by glutamate. METHODS： After being treated with picroside Ⅱ and glutamate, the viability of PC12 ceils was detected by MTT assays, and CDCFH was used to detect the reactive oxygen species （ROS）. The apoptosis rate was measured by DNA fragmentation agarose gel electrophoresis; The expression of Bcl-2 was detected by Western blot. RESULTS： Picroside Ⅱ could significantly protect PC12 ceils from the apoptosis induced by glutamate, increase cell survival rate, decrease the level of intracellular ROS and upregulate Bcl-2 protein expression. CONCLUSION： The underlying mechanisms may be involved in the inhibition or decreasing of ROS formation, and increasing the expression of Bcl-2.