灯盏花素对氯化钾诱导的培养平滑肌细胞内游离钙的影响(英文)

EFFECTS OF BREVISCAPINE ON KCL-INDUCED[CA^(2+)]I MOBILIZATION IN CULTURED VASCULAR SMOOTH MUSCLE CELLS

目的:观察灯盏花素对氯化钾诱导的培养的家兔平滑肌细胞内游离钙的影响。方法:用RF_5000荧光光度仪检测Fura-2AM负载的培养平滑肌细胞内游离钙的浓度。结果:在有细胞外钙存在时,静息条件下细胞内游离钙为330±100nmol·L-1,灯盏花素能轻度降低静息状态下细胞内游离钙,但无统计学意义。氯化钾50mmol·L-1将细胞内游离钙增加到420±104nmol·L-1,而灯盏花素10-6、10-5、3×10-5mol·L-1能进一步加强由氯化钾引起的细胞内游离钙的增加。然而,在无细胞外钙时,灯盏花素对细胞内游离钙却没有影响。结论:灯盏花素通过电压依赖性钙通道加强钙离子内流。

Objective： To study the effect of breviscapine on intracellular free Ca^2＋ concentration （[Ca^2＋]i） induced by KCI in cultured vascular smooth muscle cells （VSMC） of rabbits. Methods： VSMCs were cultured and loaded with Fura-2AM. [Ca^2＋]i was measured by fluorospectrophotometer （Shimadzu RF_5000,Japan）. Results： In the presence of extracellular calcium, the average [Ca^2＋]i in resting status was 330±100nmol·L^-1.Breviscapine could slightly decrease [Ca^2＋]i in resting status, which had no statistic significance. Depolarization induced by high KCl 50mmol·L^-1 could increase [Ca^2＋]i to 420±104nmo1·L^-1 （P〈0.05）,whereas 10^-6,10^-5,3×10^-5 mol· L^-1 of breviscapine could further enhance [Ca^2＋]i elevation induced by KCl 50mmol·L^-1 （P〈0.05）. Nevertheless, breviscapine had no significant effect on [Ca^2＋]i in the absence of extracellular calcium. Conclusion： Breviscapine can enhance Ca^2＋ influx into muscle cells through voltage-dependent calcium channels.