基质金属蛋白酶-7异常表达与子宫内膜癌发病关系的探讨

Study on the relationship btween abnormal expression of matrix metalloproteinases 7 with carcinoma of endometrium

目的:探讨MMP-7与子宫内膜癌发病及转移的关系。方法:利用免疫组化的方法观察72例子宫内膜癌病变组织、64例内膜癌前病变组织和80例正常子宫内膜中MMP-7及其天然抑制物TIMP-1的表达情况。结果:在内膜癌组、内膜癌前病变组MMP-7的阳性表达分别为87.5%(63/72)、75%(48/64),明显高于正常内膜组43.8%(35/80)(P<0.01),而两组之间无显著差异。内膜癌组和癌前病变组TIMP-1的阳性表达分别为80.6%(58/72)、65.6%(42/64),也明显高于正常内膜组38.8%(31/80)(P<0.01)。随着内膜癌临床分期的增加,MMP-7的表达明显增强,Ⅱ期以上的内膜癌MMP-7表达均明显高于Ⅰ期(P<0.05)。在不同组织学分类中,组织分化程度低的内膜癌MMP-7表达明显高于组织分化程度高者。淋巴结转移阳性的内膜癌组织,MMP-7表达高于淋巴结转移阴性的内膜癌组织(P<0.01)。而TIMP-1则与上述病理学参数无显著相关性。结论:MMP-7在内膜癌发生过程中起到了重要作用,MMP-7表达增强和TIMP-1分泌相对不足,是内膜癌浸润和转移的重要促进因素。

Objective： To study the relationship between abnormal expression of matrix metalloproteinases in 7 with carcinogenesis and progression of endometrium carcinoma. Methods： The uteruses samples were collected from 72 patients with carcinoma of＇endometrium, 64 patients with precancertissues and 80 normal human endomerium. Immunohistochemical analysib was employed to demonstrate the positive expression of the MMP-7 and TIMP-1 in these samples. Results： The positive expressions of MMP-7 were 87.5% and 75% in carcinoma of endometrium group and precancer tissues group. These expressions were significantly higher than that in normal endometrium group. The same results took place in TIMP-1. It was significantly amplified that expression of MMP-7 as raise of clinical disease period and decrease of tissue classification. The expression was higher in lymph node metastasis group than that in no lymph node metastasis group. No relationship was found between TIMP- I expression and these clincopathologic parameter. Conclusion： MMP-7 is an important role in carci- noma of endometrium development. MMP-7 increase and TIMP-1 comparative decrease are the important promotion factors in carcinoma of endometrium infiltration and transition.