响应面分析法优化地木耳多糖提取工艺的研究

Study on extraction of Nostoc commune vauch polysaccharides by response surface methodology

目的:研究地木耳多糖的水提法优化工艺。方法:采用苯酚-硫酸法测定多糖含量,以多糖得率为考察指标,在单因素试验的基础上,利用响应面分析法对地木耳多糖提取工艺进行优化研究。结果:提取温度、提取时间以及液料比与响应值地木耳多糖得率存在显著的相关性,最佳提取工艺条件:提取温度99℃,时间3.76 h,液料比52.1∶1,提取3次,在此条件下地木耳多糖得率理论值达到7.70%,验证试验的多糖得率为7.54%,地木耳多糖样品中多糖含量为53.5%。结论:该优化工艺的多糖得率高,可用于地木耳多糖的提取。

Objective： To study the extraction technology of Nostoc commune vauch polysaccharides. Methods： The content of polysaccharides of Nostoc commune vauch was determined by the method of phenol-sulphate acid. Based on the results of one-factor-at-a-time-technique method, A three-level Box-Behnken factorial design was employed combining with response surface methodology （RSM） to optimize the extraction conditions for the yield of Nostoc commune vauch polysaccharides （NCVPS）. Results： The results showed that temperature was the most important factor for the extraction percentage of the polysaccharides, time was second and ratio of liquid-solid was third. The optimal extraction technology conditions were that the temperature was 99℃, the extraction time was 3.76 h, the ratio of liquid-solid was 52.1 ： 1 and the extraction times were 3 times, the yield of the polysaccharides was 7.54%, and the content of polysaccharides was 53.5%. Conclusion： This extraction technology could be used for the extraction of Nostoc commune vauch polysaccharides （NCVPS）.