大熊猫辅酶Q-细胞色素C氧化还原酶的辅酶Q连接蛋白基因cDNA的克隆及序列比较

cDNA cloning and sequences analysis of ubiquinol-cytochrome c reductase complex ubiquinone-binding protein (QP-C) from giant panda

In order to provide scientific material for promulgating the giant panda(Ailuropoda melanoleuca)nucleus gene inheritance characteristic,for the formulation gene protection countermeasure and to put resources to rational use,the expression sequence of ubiquinol-cytochrome c reductase complex ubiquinone-binding protein(QP-C)gene from the giant panda was analyzed.The result indicates that the cDNA sequence length is 335 bp and contains a 246-nucleotide open reading frame encoding 81 amino acid residues.The pI of the protein is 10.50 and its mole cular weight is 9.60×103 Da.Topology prediction shows these QP-C proteins contain a N-glycosylation site,a potential protein kinase C phosphorylation site and a Casein kinase II phosphorylation site.Comparing the cDNA sequence with amino acid sequence of giant panda’s QP-C gene and cDNA sequence with the amino acid sequence of other species’ QP-C gene shows high similarity.The similarity is 82.3% to 95.2% and 81.5% to 95.1%.

In order to provide scientific material for promulgating the giant panda （Ailuropoda melanoleuca ） nucleus gene inheritance characteristic, for the formulation gene protection countermeasure and to put resources to rational use, the expression sequence of ubiquinol-cytochrome c reductase complex ubiquinone-binding protein （ QP-C ） gene from the giant panda was analyzed. The result indicates that the cDNA sequence length is 335 bp and contains a 246-nucleotide open reading frame encoding 81 amino acid residues. The pI of the protein is 10. 50 and its mole cular weight is 9.60 × 10^3 Da. Topology prediction shows these QP-C proteins contain a N-glyeosylation site, a potential protein kinase C phosphorylation site and a Casein kinase Ⅱ phosphorylation site. Comparing the cDNA sequence with amino acid sequence of giant panda ＇ s QP-C gene and cDNA sequence with the amino acid sequence of other species＇ QP-C gene shows high similarity. The similarity is 82. 3% to 95.2% and 81.5% to 95. 1%.