摘要
In order to provide scientific material for promulgating the giant panda(Ailuropoda melanoleuca)nucleus gene inheritance characteristic,for the formulation gene protection countermeasure and to put resources to rational use,the expression sequence of ubiquinol-cytochrome c reductase complex ubiquinone-binding protein(QP-C)gene from the giant panda was analyzed.The result indicates that the cDNA sequence length is 335 bp and contains a 246-nucleotide open reading frame encoding 81 amino acid residues.The pI of the protein is 10.50 and its mole cular weight is 9.60×103 Da.Topology prediction shows these QP-C proteins contain a N-glycosylation site,a potential protein kinase C phosphorylation site and a Casein kinase II phosphorylation site.Comparing the cDNA sequence with amino acid sequence of giant panda’s QP-C gene and cDNA sequence with the amino acid sequence of other species’ QP-C gene shows high similarity.The similarity is 82.3% to 95.2% and 81.5% to 95.1%.
In order to provide scientific material for promulgating the giant panda (Ailuropoda melanoleuca ) nucleus gene inheritance characteristic, for the formulation gene protection countermeasure and to put resources to rational use, the expression sequence of ubiquinol-cytochrome c reductase complex ubiquinone-binding protein ( QP-C ) gene from the giant panda was analyzed. The result indicates that the cDNA sequence length is 335 bp and contains a 246-nucleotide open reading frame encoding 81 amino acid residues. The pI of the protein is 10. 50 and its mole cular weight is 9.60 × 10^3 Da. Topology prediction shows these QP-C proteins contain a N-glyeosylation site, a potential protein kinase C phosphorylation site and a Casein kinase Ⅱ phosphorylation site. Comparing the cDNA sequence with amino acid sequence of giant panda ' s QP-C gene and cDNA sequence with the amino acid sequence of other species' QP-C gene shows high similarity. The similarity is 82. 3% to 95.2% and 81.5% to 95. 1%.
出处
《兽类学报》
CAS
CSCD
北大核心
2007年第2期190-194,共5页
Acta Theriologica Sinica
基金
国家自然科学基金资助项目(30570275)
四川省应用技术项目(2006J13-057)
四川省教育厅重点科研项目(2004A101)
四川省重点学科建设项目(SZD0420)
关键词
大熊猫
RT-PCR
辅酶Q连接蛋白基因
克隆
Cloning
Giant panda ( Ailuropoda melanoleuca )
RT-PCR
Ubiquinone-binding protein