紫芝超细粉挥发油成分GC-MS分析

GC-MS analysis of chemical components in the essential oils of from the ultrafine-powder of Ganoderma sinense

将紫芝Ganoderma sinense(“闽紫96”,HMAS 77207,阔叶树枝桠材或边材栽培)超微粉碎加工成500目的子实体超细粉。激光粒度分析表明:紫芝超细粉的表面积到834．18m^2/kg,中位径(D50)为12．68μm。气质联用分析结果表明:从紫芝超细粉挥发油中分离到35种成分,出峰时间为7．872～30．239min,确定了其中32个成分,占相对含量的88．642％;绝大多数成分为脂肪族化合物(67．216％),主要成分(20．67％)为2,6-二叔丁基一苯酚为芳香族化合物,还检出2种杂环族化合物。

The fruitbodies of Ganoderma sinense （＂Minzi 96＂ strain, HAMS 77207, cultivated with laburnum or branches of broadleaf tree） were processed into ultrafine-powder that sifted through φ500 R40/3 sieve. And the granularity were tested by laser grain-size analysis, that specific surface area （BET）was 834.18m^2/kg, and the cumulative distribution data D50 was 12.68μm（equivalent grain-size）. 35 compositions of total essential oil from the ultrafine-powder were separated and identified by GC-MS, retention time arranged from 7.872 min to 30.239min. The results indicated that 32 compounds were determinated, and their relative contents were about 88.64%, most of the compounds belong to aliphatic compounds （67.216%）, and the main constituent with highest relative content （20.67%） have been identified as 2,6-di-tert-butylpbenol（aromatics compounds）, moreover, two compounds of heterocyclics were also checked out.