维吾尔族、汉族慢性胃炎患者中HLA-DRB1*12等位基因与幽门螺杆菌感染的关系

The association of the helicobacter pylori infection and HLA-DRB1 alleles in Uygur ethnic in Xinjiang

目的:探讨慢性胃炎患者HLA-DRB1*12等位基因与幽门螺杆菌(Hp)感染的关系。方法:采用Hp分离培养技术检测36例汉族、33例维吾尔族慢性胃炎患者的Hp感染情况,并采用聚合酶链反应-序列特异性引物(PCR-SSP)方法检测HLA-DRB1*12等位基因多态性。结果:汉族、维吾尔族慢性胃炎患者Hp阳性率分别为56%、79%,维吾尔族明显高于汉族(P<0.05);维吾尔族与汉族慢性胃炎患者Hp(-)者的HLA-DRB1*12等位基因检出率高于Hp(+)者,但差异无统计学意义(P>0.05)。结论:维吾尔族慢性胃炎患者Hp阳性率可能高于汉族,维吾尔族与汉族慢性胃炎患者中HLA-DRB1*12等位基因可能与Hp感染无关。

Objective.. To compare the prevalence of H. pylori infection in gastritis of Xinjiang Uygur, Han ethnic groups, and to analyze the association of HLA-DRB1 ＊ 12 allele of these two ethnic groups gastritis with H. pylori infection. Methods： Diagnosis by H. pylori culture from gastric mucous for 36 Han and 33 Uygur chronic gastritis. Polymorase chain reaction single strand polymorphism （PCR-SSP） technique was applied to determine the polymorphism of the HLA-DRB1 ＊ 12 locus of these groups and then the association of HLA-DRB1 ＊ 12 allele of these groups with H. pylori infection was analyzed by SPSS software. Resuits： The prevalence of H. pylori infection was 56% in Han patients which was lower than that in Uygur patients （79%）, （P 〈0.05）. The distribution of HLA-DRB1＊ 12 locus was not significantly different between the 2 groups. The frequency of HLA-DRB1 ＊ 12 allele was higher in person without H. pylori infection （P〉0.05）. Conclusions. The results indicated that the prevalence of H. pylori infection in Uygur was higher than in Han ethnic group. HLA-DRB1 ＊ 12 allele have no relationship with H. pylori infection.