甲醛对小鼠血清IgG、IgM和白细胞介素-4合成水平的影响

Effect of formaldehyde on the concentration of peripheral blood serum IgG,IgM and IL-4 in mice

目的:探讨甲醛对小鼠外周血免疫球蛋白(IgG、IgM)、细胞因子白细胞介素-4(IL-4)合成水平的影响。方法:80只成年昆明种小鼠随机分成对照组及甲醛低、中、高剂量组,对照组除不给甲醛外,其他处理同实验组,低、中、高剂量组每天2h吸入不同浓度气态甲醛(0、2.5、10及30mg/m3),持续2周,采用ELISA法测定各组小鼠外周血清中IgG、IgM及IL-4水平。结果:外周血中IgG、IgM的表达随甲醛浓度的升高而增加,高剂量组明显高于对照组和低剂量组,差异有统计学意义(P<0.05);IL-4的水平亦随甲醛暴露浓度的升高而升高,中、高剂量组均显著高于对照组,差异有统计学意义(P<0.05)。结论:甲醛吸入使小鼠体内IgG、IgM和IL-4水平升高,并有诱导变态反应和哮喘发生的可能。

Objective： To explore the effect of formaldehyde on peripheral blood Immunoglobulin G, M, and Interleukin-4 synthesis level. Methods： Eighty KM mice were randomly divided into controlled group and 2.5 mg/m^3 , 10 mg/m^3 , 30 mg/m^3 concentration by inhalation formaldehyde （FA） for 2h per day and continuously for 14 days. The concentration of peripheral blood serum IgG, IgM and IL-4 were determined. Results： A significant increase tendency of the express on peripheral blood serum IgG, IgM was showed with increasing of doses. There was significant difference in 30 mg/m^3 group FA inhalation groups compared with control group and 2.5 mg/m^3 group （P 〈0.05）. The IL-4 concentrations of peripheral blood serum were increased significantly accompanying the augment of FA concentration. The incorporation of 10 and 30 mg/m^3 FA group was significantly higher than that of control group, the difference was statistical significance （P 〈0.05）. Conclusion： Formaldehyde can induce the results that were the higher concentrations of IgG, IgM in peripheral blood serum. The results suggest that formaldehyde may induce the originated chance of allergies and asthma.