人β防御素-2基因在解脲支原体感染女性生殖道组织中的表达

Expression of human beta-defensin 2 in female genital tract with Mycoplasma infection

目的:检测人β防御素-2基因(hBD-2)在正常及解脲支原体感染的女性生殖道的表达情况及与炎症程度的关系。方法:采用逆转录聚合酶链反应(RT-PCR)检测正常及解脲支原体感染的女性宫颈、子宫内膜中hBD-2基因的表达情况,用β-actin作内参照。结果:在正常及解脲支原体感染女性宫颈、子宫内膜中hBD-2基因均有表达,且支原体感染的女性hBD-2基因表达比正常女性明显增强,二者差异有统计学意义(P<0.01)。结论:hBD-2在正常及解脲支原体感染的女性生殖道中有广泛表达,且后者比前者明显,提示hBD-2在正常女性生殖道特别是在解脲支原体感染中起着重要的防御作用。

Objective： To examine Human β - defensin 2 （ hBD - 2） mRNA expression in female genital tract of normal and with Mycoplasma infection, Method： The experiments of hBD - 2 mRNA expression were perfo .r.med by reverse transcription - polymerase chain reaction（ RT - PCR） with the samples from female genital tract of normal and with Mycoplasma infection and the β - actin was used as positive control. Results ： The study revealed that hBD - 2 mRNA expressed in all female genital tract of normal and with Mycoplasma infection was taken from cervix and endometrium, but the expression in women with Mycoplama infection is much more than that of normal women, there was significant difference by statistical analysis. Conclusions ： hBD - 2 plays an important role of defence in female genital tract of normal and especially with Mycoplama infection,