EGCG对大鼠肾脏缺血再灌注损伤的保护作用

The protective effect of epigallocatechin gallate on renal ischemia-reperfusion injury in rats

目的研究表绿茶活性成分没食子儿茶素-3-没食子酸酯(epigallocatechin gallate,EGCG)对大鼠肾脏缺血再灌注损伤是否具有保护作用。方法将12只Lewis大鼠随机分为2组:(1)实验组大鼠7只,于肾缺血后的再灌注期将10 mg/kg的EGCG置于2ml生理盐水中静脉注射;(2)对照组大鼠5只,于肾缺血后的再灌注期静脉注射2 ml生理盐水。2 h后处死动物,收集血液和肾脏标本,行血肌酐(Cr)、尿素氮(BUN)、肾组织超氧化物歧化酶(SOD)和脂质过氧化产物丙二醛(MDA)含量测定,并进行组织病理和超微病理检查。结果实验组大鼠血Cr和BUN水平明显低于对照组(P<0.05),肾组织中SOD活性较对照组明显升高(P<0.01),肾组织脂质过氧化产物MDA的含量较对照组明显降低(P<0.01)。病理学检测表明实验组大鼠肾小管周围毛细血管内未见淤血,肾小管上皮细胞变性及线粒体的损伤减轻。结论EGCG对大鼠肾脏缺血再灌注损伤具有保护作用。

Objective To study the protective effect of epigallocatechin gallate （EGCG） on renal ischemia-reperfusion injury in rats. Methods Twelve Lewis rats were divided into 2 groups： the experimental group （n = 7） was intravenously administered with ECJCG at the dose of 10 mg/kg during the reperfusion period, and the remaining rats served as control group. At the end of reperfusion period, all the rats were sacrificed. Blood and kidney samples were collected for the blood levels of creatinine and urea nitrogen, the tissue concentration of malondialdehyde （MDA） and superoxide dismutase （SOD） and pathological studies. Results The blood levels of creatinine and urea nitrogen in experimental group were lower than in control group （P〈0. 05）. The tissue concentrations of MDA and SOD in experimental group was reduced and increased, respectively, as compared with control group （P〈0. 01）. Pathological studies indicated that EGCG could attenuate the ischemia-reperfusion injury in rats. Conclusion EGCG is a promising agent, which can be implicated into the therapy for ischemia and reperfusion injury.