砷和香烟烟气溶液对氧化应激的协同作用

Oxidative stress in rat lymphocytes induced by arsenite and cigarette smoke solution

目的研究亚砷酸钠和香烟烟气溶液联合作用对大鼠淋巴细胞氧化应激的影响,并探讨两者对氧化应激的影响是否存在交互作用。方法大鼠淋巴细胞分为4组:亚砷酸钠单独作用组、香烟烟气溶液单独作用组、两者联合作用组和对照组,染毒后用流式细胞术检测细胞内活性氧的含量,用微量荧光法检测细胞内丙二醛含量,用彗星实验检测细胞的DNA损伤,同时采用2×2析因设计研究两者的交互作用。结果亚砷酸钠单独作用组、香烟烟气溶液单独作用组和两者联合作用组细胞内的活性氧含量、丙二醛含量、彗星尾长和细胞拖尾率均高于对照组。析因设计分析结果表明两者对细胞内活性氧含量、丙二醛含量及彗星尾长的影响存在交互作用。结论亚砷酸钠和香烟烟气溶液对大鼠淋巴细胞氧化应激的影响存在交互作用,交互作用方式为协同作用。

Objective To investigate oxidative stress in rat lymphocytes induced by arsenite and cigarette smoke solution and study their interaction. Methods Rat lymphocytes were divided into 4 groups： sodium arsenite treated group, cigarette smoke solution treated group, cigarette smoke solution and sodium arsenite treated group and control group. After the exposure, ROS production was detected by flow cytometry; Intracellular malondialdehyde was detected by fluorometry; DNA damage was detected by comet assay. 2 × 2 factorial design was used to evaluate the interaction. Results ROS, intracellular malondialdehyde, length of comet tail and tailed cell percentage in sodium arsenite treated group, cigarette smoke solution treated group, cigarette smoke solution and sodium arsenite treated group were significantly higher than those in control group. The results of the factorial design showed ： Sodium arsenite and cigarette smoke solution had interaction on ROS production, malondialdehyde levels and length of comet tail, the interaction was synergism. Conclusions Sodium arsenite and cigarette smoke solution have interaction on oxidative stress in rat lymphocytes, and the interaction is synergism.