Connexin 43在神经原性逼尿肌过度活动中表达的实验研究

Expression of Connexin 43 in Overactive Neurogenic Detrusor

目的研究Connexin 43在神经原性逼尿肌中的表达情况。方法选择神经原性逼尿肌过度活动(neurogenic detrusor overactivity,NDO)患者16例为Ⅰ组,压力性尿失禁(stress urinary incontinence,SUI)膀胱逼尿肌功能正常患者10例为Ⅱ组,取两组逼尿肌标本,利用免疫组织化学和RT-PCR技术检测两组逼尿肌细胞中Connexin 43及其蛋白表达变化情况。结果免疫组织化学及RT-PCR技术检测结果显示,Ⅰ组逼尿肌细胞中Connexin 43及其蛋白含量明显高于Ⅱ组(P(0.05)。结论神经原性逼尿肌过度活动逼尿肌中Connexin 43基因表达增加,可能与神经原性逼尿肌过度活动的发生有密切关系。

Objective To investigate the expression changes of connexin 43 among the neurogenic detrusor overactivity. Methods Detrusor biopsies have been obtained from open operation evaluated patients with neurogenic detrusor overactivity（NDO） （n = 16） （group Ⅰ ） ,and from patients with stress urinary incontinence（SUI） with stable, non -obstructed detrusors （n =10） （groupⅡ ）. Connexin 43 mRNA and it＇s proteinum levels were evaluated using quantitative RT - PCR and Immunohistochemistry method. Results By using quantitative RT - PCR and Immunohistochemistry method, we find the expression of connexin 43 mRNA and proteinum in group Ⅰ was much higher than that in group Ⅱ （ P 〈 0.05 ）. Conclusion The expression of connexin 43 gene was significantly increased, which might have a close relationship with neurogenic detrusor overactivity.