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干扰素基因定向进化库的建立及初步筛选 被引量：1

Established and Screened of Directed Evolution Library of Interferon Gene in Swine
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摘要 目的构建α干扰素基因家族定向文库。方法利用DNA shuffling技术对人、鸡和猪α干扰素基因进行人工改造,结合噬菌体展示技术构建噬菌体基因改组文库,并对文库进行验证。结果构建了α干扰素基因家族定向文库,其容量为3.5×107pfu;测序得到了3条与猪、人α干扰素基因高度同源的序列。结论得到了1个高质量的α干扰素基因家族定向文库,为今后筛选高效的α干扰素奠定了基础。 Objective To construct a library of α-interferon gene. Methods A library of human, swine, and avian interferon gene was constructed using both DNA shuffling technique and phage display technology. Results The titers of un-amplified and the amplified library are 3.5 ×10^7 pfu/ml,respectively. 3 of the human interferon DNA sequences show a high degree of homology with pig interferon. Conclusion A high quality interferon gene library was constructed successfully.
作者 吴明福 蒋忠军 马玉海 唐博恒 王君伟
机构地区 东北农业大学 广州军区疾病预防控制中心
出处 《热带医学杂志》 CAS 2007年第5期426-428,共3页 Journal of Tropical Medicine
基金 广州市科技攻关项目(No.200223-B4022)
关键词 Α干扰素 DNA改组 文库 α-interferin DNA shuffling library
分类号 R373 [医药卫生—病原生物学]
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参考文献10

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共引文献14

同被引文献33

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热带医学杂志
2007年 第5期

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