脂血标本的核酸定量检测效果评价

Evaluation of Lipoid Serum Samples' DNA Quantification through FQ-PCR

目的评价脂血标本的核酸定量检测实时扩增曲线,以探讨此类标本进行HBV DNA定量检测的可接受性。方法建立扩增曲线观察法、扩增效率Grubbs离散值分析法及扩增效率置信区间估计法,评价接收脂血标本进行荧光定量PCR法HBV DNA定量检测的有效性。结果该2批次扩增的各样品扩增效率数值未发现离散值,3例脂血标本的扩增效率在同批样品扩增效率的90%置信区间内,与扩增曲线观察法结果一致。结论3种评价方法均对修正标准操作程序以接收该类标本提供了支持性的依据。

Objective To evaluate the feasibility of using real-time FQ-PCR for quantitative measurement of HBV-DNA in lipid containing serum samples. Methods Three different parameters, namely amplification curves, Grubbs outlier test and confidence intervals were used to evaluate the feasibility of real-time FQ-PCR in the quantitative measurement of HBV-DNA. Results The efficiency of amplification in two trial runs was similar. Amplification efficiency of the 3 serum samples was in 90% confidence range. The results were consistent with the amplification curves. Conclusion These three parameters are useful in amending the standard operation procedures in the amplification HBV- DNA in lipid containing serum.