一丁基锡和二丁基锡致小鼠骨髓细胞DNA损伤

Effects of monobutyltin or dibutyltin on DNA damage of mice bone marrow cells

目的研究不同剂量的一丁基锡、二丁基锡对体外培养的小鼠骨髓细胞DNA损伤作用。方法应用彗星试验检测不同浓度一丁基锡、二丁基锡对小鼠骨髓细胞DNA损伤。结果结果1×10-10,1×10-11g/L剂量的一丁基锡、二丁基锡与对照组比较,差异有统计学意义(P<0.05,P<0.01)。随着剂量的增加,DNA损伤也越严重,存在着明显的剂量-效应关系。组间比较,差异有统计学意义。结论体外条件下,一丁基锡、二丁基锡可引起小鼠骨髓细胞DNA的损伤。

Objective To study the effects of monobutyltin or dibutyltin on DNA damage of mice bone marrow cells in vitro. Methods The DNA damage was observed by means of comet assay. Results Compared with the control group, the treated groups of 1 × 10^ - 10,1 × 10^ - 11 g/L monobutyltin or dibutyltin were significantly different（ P 〈 0.05, P 〈 0.01 ）. The DNA damage increased with the increase of dose and there was significant dose-effect relationship. Significant difference was found among the treated groups. Conclusion Monobutyltin or dibutyltin could cause DNA damage of mice bone marrow cells in vitro.