姜黄素对沙土鼠脑缺血/再灌注损伤的海马CA1区细胞凋亡和即早基因c-fos、c-jun、NF-κB表达变化的关系

THE RELATIONSHIP BETWEEN THE EFFECTS OF CURCUMIN ON CEREBRAL ISCHEMIA/REPERFUSION INJURY AND IMMEDIATELY GENIC EXPRESSIONS OF Fos, Jun and NF-κB IN HIPPOCAMPAL CA1 AREA AND ITS SIGNIFICANCE IN GERBILS

目的探讨姜黄素对沙土鼠海马CA1区缺血/再灌注损伤的影响及与即早基因c-fos、c-jun、NF-κB在海马CA1区表达的关系及意义。方法采用沙土鼠双侧颈总动脉阻断缺血/再灌注损伤模型。动物随机分为假手术组(SH)、脑缺血/再灌注组(I/R)、姜黄素组(CU)、溶剂对照组(SC);每组据再灌注时间点的不同又分多个亚组,每组6只动物。在预定时间点行开阔法行为学检查、TUNEL法海马CA1区凋亡细胞检测,免疫组织化学ABC法测定c-fos、c-jun、NF-κB蛋白在海马CA1区的动态变化。结果姜黄素可显著减少沙土鼠探索活动及海马CA1区凋亡锥体细胞数量(P<0.01)、诱导Fos蛋白及抑制Jun和NF-KB蛋白的表达(P<0.01)。结论姜黄素具有脑保护作用,调控即早基因c-fos、c-jun和NF-κB的表达可能是作用机制之一。

To explore the relationship between the effects of curcumin on cerebral ischemic/reperfusion injury and immediately genic expressions of Fos, Jun and NF-κB in hippocampal CA1 area. Methods： Gerbils were randomly divided into sham group （SH）, is-chemia/reperfusion group（I/R）, curcumin group （CU） and .solvent control group（SC）. Forebrain ischemia was induced by occlusion of bilateral common carotid arteries. Observations were carried out in each group 15 min, 1h,2h,6h, 1d, 3d, 5d and 7d after is-chemia： open field test was used to examine the behavioral change, the apoptosis neurons in hippocampal CA1 region was counted, the expression of Fos, Jun and NF-κB in hippocampal CA1 was detected by SABC immunocytochemical technique. Results： The behav-ioral mark and the number of apoptosis neurons in hippocampal CA1 region was much less in CU group than in I/R group（ P〈 0.01 ） The expression of Fos was more and the expression of Jun and NF-κB was less in CA1 area in CU group than in I/R group（P〈 0.01 ）. Conclusion： Curcumin can significantly protect neurons against cerebral ischemia, increasing the expression Fos and decreas-ing the expression of Jun and NF-κB may be the protective mechanisms.