急性力竭运动对大鼠胃肠传输速率及回肠肌间神经丛氮能神经的影响

EFFECT OF ACUTE EXHAUSTIVE EXERCISE ON GASTROINTESTINAL TRANSIT RATE AND NITRIERGIC NERVES IN MYENTERIC PLEXUS IN RATS ILEUM

目的研究力竭运动对大鼠胃肠动力的影响及其肠神经机制。方法24只大鼠随机分成对照组和急性力竭运动组,建立力竭运动大鼠模型,测定胃肠传输速率,用酶组织化学方法和计算机图像分析技术对两组大鼠回肠肌间神经丛内氮能神经元的数目和一氧化氮合酶(NOS)的表达进行测定。结果急性力竭运动组大鼠胃肠传输速率明显延迟,回肠肌间神经丛内氮能神经元的数目明显增多和NOS的表达显著增强(P<0.05和P<0.01)。结论大鼠力竭运动后小肠肌间神经丛内氮能神经元的数目增多和NOS的表达增强可能是导致胃肠传输速率延迟的重要原因之一。

To investigate the effect of acute exhaustive exercise on gastrointestinal motility and its enteric nervous mechanisms. Methods： 24 rats were randomly divided into control group（C） and acute exhaustive exercise group（AEE）. The rate of gastrointesti-nal transit was measured and histologic changes of nitriergic nerves in ileum myenteric plexus were observed with enzymatic histo-chemical and image analytic technique. Results： In the rats of AEE group, the rate of gastrointestinal transit was ddayed comparing with C group（P〈0.05）, the numbers of nitriergic neurons and expression levels of nitric oxide synthase（NOS） in the ileum myen-teric plexus significantly increased comparing with C group（ P〈 0.01 ）. Conclusion： It is possible that increase of nitriergic neurons and expression levels of NOS in the myenteric plexus of small intestine are one of the mechanisms of dday of gastrointestinal transit rate in acute exhaustive exercise rats.