谭清苏铁性别相关的RAPD标记研究

Identification of a Sex-associated RAPD Marker in Cycas tanqingii D. Y. Wang

以谭清苏铁(Cycas tanqingii D.Y.Wang)雌雄植株半年生羽叶为材料,用优化的CTAB法分别提取其全基因组DNA,进行RAPD单因子梯度实验和正交实验以优化扩增条件。应用160个RAPD随机引物检测基因组DNA,雌雄植株均扩增出1450多条带,其中引物S0465扩增出与谭清苏铁雌株高度相关的RAPD标记,其大小约为500bp,该标记与雄株没有关联。

The total genomic DNA was extracted from half-a-year-old leaflets of the male and female cycads （Cycas tanqingji D. Y. Wang） using an optimized CTAB protocol. The RAPD-PCR amplification conditions were optimized by monofactor and multifactor orthogonal experiments. A total of 160 primers were used for RAPD-PcR amplification and more than 1 450 fragments were obtained from both male and female plants. A fragment of about 500 bp only linked to the female was generated by the S0465 primer, which can be used in sex determination of C. tanqingji.