CD105和MMP-2在子宫腺肌病组织中的表达及意义

Expression and Significance of MMP-2 and CD105 in Adenomyosis

目的探讨以CD105标记的微血管密度（MVD）与MMP-2在子宫腺肌病中的表达与发病机制的关系。方法收集我院2005-01～2006-01间治疗的30例子宫腺肌病病例,采用免疫组织化学染色检测子宫腺肌病在位组及异位组子宫内膜CD105、MMP-2的表达,并运用图像分析仪检测CD105标记的MVD。结果以CD105标记的MVD异位组为24.19±5.30,在位组为17.89±4.53,异位组子宫内膜中表达显著高于在位组子宫内膜（P〈0.05）,在位组子宫内膜分泌期MVD18.09±3.60、增生期17.69±5.57,无显著差异（P〉0.05）;MMP-2在子宫腺肌病异位组内膜的阳性表达率76.67%（23/30）,在位组阳性表达率为46.67%（14/30）,异位组内膜的表达水平显著高于在位组子宫内膜（P〈0.05）,在位组子宫内膜分泌期MMP-2的阳性表达率显著高于增生期（P〈0.05）。经过spearman相关性分析,MMP-2阳性率的表达与MVD呈显著正相关关系（r=0.489,P〈0.01）。结论CD105和MMP-2的过度表达与子宫腺肌病新生血管的生成有关,在子宫腺肌病发病过程中发挥重要作用,是子宫腺肌病具有侵袭性生长的原因之一。

Objective To investigate the expression of MMP-2 and CD105 in the eutopie and ectopie endometrium of adenomyosis and their relation with pathogenesis of adenomyosis. Methods Enometrial specimens were obtained from 30 cases with adenomyosis. MMP - 2 were detected by immtmohistochemical taining, microvessels were highlighted by immtmohistochemical staining by detected CD105. the average microvessels counted per field（400 × ） were obtained by use of a computerized image analysis system. Results The expression of MVD in the ectopic endometrium and in the dutopic endometrium of adenomyosis tissues were 24.19 ± 5.30,17.89 ± 4.53, the expression of MVD in two group above had significant difference （ P 〈 0.05）, The expression of MVD in the eutopic endometrium of secretory phase was not significantly higher than that of proliferative phase （ P 〉 0.05） ;The positive expression of MMP- 2 in the eetopic endometrium and in the entopic endometrium were 76.67%（23/30） and 46.67%（14/30）.The expression of MMP-2 in the eetopic endometrium of adenomyosis was significantly higher than that in the eutopic endometrium of adenomyosis （ P 〈 0.05） .The expression of MMP- 2 in the eutopic of adenomyosis of secretory phase was signiflcandy higher than that of proliferative phase（ P 〈 0.05） ;MVD was significandy correlated with the expression of MMP-2（ r =0.489, P 〈 0.01）.Conclusion The overexpression of MMP-2 and MMP-2 significantly have close relationship with angiogenesis of adenomyosis which play an important role in adenomyosis, and participate in the process of invasion.